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AB152123

Anti-RFP 抗体

Anti-RFP antibody

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(3 Publications)

Rabbit Polyclonal RFP antibody. Suitable for IP, WB, ICC/IF and reacts with Tag samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP.

別名を表示する

Red fluorescent protein drFP583, DsRed

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (AB152123)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RFP antibody (AB152123)

Immunofluorescence analysis of RFP-transfected HeLa cells labeling RFP with ab152123 : (1) RFP is expressed in the tranfected cell, (2) The cell expressing RFP is detected using ab152123 at 1/5000 followed by Alexa Fluor 488 at 1/500 Goat anti-Rabbit IgG, (3) Merged with DNA probe, the lower cell represents a negtive control.

Immunoprecipitation - Anti-RFP antibody (AB152123)
  • IP

Unknown

Immunoprecipitation - Anti-RFP antibody (AB152123)

Immunoprecipitation of RFP-tagged protein.
HeLa transfected lysates were subjected to immunoprecipitation using :
Lane 1 : 15 μg of HeLa transfected lysate
Lane 2 : normal rabbit IgG
Lane 3 : 2.5 μg ab152123
Detection ab152123 at 1/10000 dilution

All lanes:

Immunoprecipitation - Anti-RFP antibody (ab152123)

Predicted band size: 26 kDa

false

Western blot - Anti-RFP antibody (AB152123)
  • WB

Unknown

Western blot - Anti-RFP antibody (AB152123)

12% SDS PAGE

All lanes:

Western blot - Anti-RFP antibody (ab152123) at 1/10000 dilution

Lane 1:

Non-transfected 293T whole cell lysate at 5 µg

Lane 2:

GFP-transfected 293T whole cell lysate at 5 µg

Lane 3:

RFP-transfected 293T whole cell lysate at 5 µg

Predicted band size: 26 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

アプリケーション

ICC/IF, WB, IP

applications

免疫原

Recombinant Full Length Protein corresponding to Sea anenome RFP_DISSP.

Q9U6Y8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5000", "ICCIF-species-notes": "<p></p>" } } }
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下記製品もご検討ください

AB185921

Anti-RFP antibody [EPR18992]

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0 Reviews
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こちらの製品も推奨する理由

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts.
See full target information RFP_DISSP
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 95:e0015321 PubMed34287041

2021

Orf Virus ORF120 Protein Positively Regulates the NF-κB Pathway by Interacting with G3BP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlong Zhou,Jiyu Guan,Feng Gao,Zi Li,Yungang Lan,Huijun Lu,Deguang Song,Lijun Lv,Pin Lv,Mengshi Xu,Zhenzhen Wang,Hongbin He,Kui Zhao,Wenqi He

Theranostics 10:8446-8467 PubMed32724480

2020

Lentivirus-mediated IL-10-expressing Bone Marrow Mesenchymal Stem Cells promote corneal allograft survival upregulating lncRNA 003946 in a rat model of corneal allograft rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiao Lu,Yusha Ru,Chenchen Chu,Ying Lv,Yichen Gao,Zhe Jia,Yue Huang,Yan Zhang,Shaozhen Zhao

eLife 6: PubMed28425915

2017

Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryos.

Applications

IP

Species

Unspecified reactive species

Shai R Joseph,Máté Pálfy,Lennart Hilbert,Mukesh Kumar,Jens Karschau,Vasily Zaburdaev,Andrej Shevchenko,Nadine L Vastenhouw
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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