JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB182145

Anti-RFC4 抗体 [EPR14556]

Anti-RFC4 antibody [EPR14556]

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Recombinant Monoclonal RFC4 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

別名を表示する

Replication factor C subunit 4, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC37, RFC4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EPR14556] (AB182145)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EPR14556] (AB182145)

Immunofluorescent staining of HepG2 cells(right) using anti-RFC4 Alexa Fluor® 555 RabMAB and HepG2 cells (left) using Dapi (blue)

Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (AB182145)
  • IP

Supplier Data

Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (AB182145)

Detection of Human RFC4 by Western Blot of Immunoprecipitate. HeLa whole cell lysate loaded, ab182145 used for blotting immunoprecipitated RFC4 at a 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-RFC4 antibody [EPR14556] (ab182145)

Predicted band size: 40 kDa

false

Western blot - Anti-RFC4 antibody [EPR14556] (AB182145)
  • WB

Supplier Data

Western blot - Anti-RFC4 antibody [EPR14556] (AB182145)

All lanes:

Western blot - Anti-RFC4 antibody [EPR14556] (ab182145) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 20 µg

Lane 4:

K562 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 40 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14556

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IP, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The replication factor C subunit 4 also known as RFC4 is an essential protein in DNA replication possessing a molecular weight of approximately 37 kDa. RFC4 forms part of the larger replication factor C (RFC) complex which functions as a clamp loader for the proliferating cell nuclear antigen (PCNA). This activity facilitates the attachment of PCNA to the DNA promoting DNA polymerase processivity during replication. RFC4 is widely expressed across various tissues with higher levels noted in proliferating cells.
Biological function summary

RFC4 plays a critical role in maintaining genomic stability and facilitating proper cell cycle progression. As a component of the RFC complex it cooperates with additional subunits to engage with DNA replication machinery effectively. This complex recognizes the primer-template junction and recruits PCNA to assist DNA polymerase in duplicating the genome accurately. RFC4's role does not end with replication as the RFC complex also partakes in DNA repair mechanisms needed for correcting replication errors.

Pathways

RFC4 is actively involved in the DNA replication pathway and the DNA damage response pathway. Within these pathways it interacts with proteins like DNA polymerase delta and epsilon ensuring their efficient function during DNA synthesis and repair. RFC4 collaborates closely with PCNA in these pathways to maintain fidelity in replication and in responding to DNA damage highlighting its functional importance in genetic information preservation.

RFC4 holds significance in the context of cancer and genomic instability disorders. Dysregulation or mutations in RFC4 can lead to defective DNA replication and repair contributing to uncontrolled cell proliferation seen in cancer. Its interaction with other proteins such as PCNA and DNA polymerases can become aberrant further exacerbating disease progression. Research into RFC4 offers potential insights into therapeutic strategies that target replication and repair pathways for disease intervention.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA template.
See full target information Replication factor C subunit 4
style
left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10496 PubMed39627214

2024

ATAD5-BAZ1B interaction modulates PCNA ubiquitination during DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yeongjae Kim,Na Young Ha,Mi-Sun Kang,Eunjin Ryu,Geunil Yi,Juyeong Yoo,Nalae Kang,Byung-Gyu Kim,Kyungjae Myung,Sukhyun Kang

Nature communications 10:2420 PubMed31160570

2019

Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Sun Kang,Eunjin Ryu,Seung-Won Lee,Jieun Park,Na Young Ha,Jae Sun Ra,Yeong Jae Kim,Jinwoo Kim,Mohamed Abdel-Rahman,Su Hyung Park,Kyoo-Young Lee,Hajin Kim,Sukhyun Kang,Kyungjae Myung

Cell death & disease 9:259 PubMed29449545

2018

Transcriptional repression of DNA repair genes is a hallmark and a cause of cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Collin,Anda Huna,Marine Warnier,Jean-Michel Flaman,David Bernard

Journal of translational medicine 12:320 PubMed25407051

2014

Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Xiang,Lekun Fang,Yanxin Luo,Zuli Yang,Yi Liao,Ji Cui,Meijin Huang,Zihuan Yang,Yan Huang,Xinjuan Fan,Huashe Wang,Lei Wang,Junsheng Peng,Jianping Wang
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB96852

Anti-RFC4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody (AB96852)

  • 0
  • 0
Primary Antibodies

AB192021

Anti-RFC4 antibody [EP14557] - C-terminal

RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - C-terminal (AB192021)

  • 5
  • 1
Primary Antibodies

AB156780

Anti-RFC4 antibody [OTI1A8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RFC4 antibody [OTI1A8] (AB156780)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB129016

Anti-TCP1 epsilon/CCT5 antibody [EPR7562]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)

  • 5
  • 1
Primary Antibodies

AB247838

Anti-HUS1 antibody [EPR5132] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-HUS1 antibody [EPR5132] - BSA and Azide free (AB247838)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB251705

Anti-TMED2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMED2 antibody (AB251705)

  • 0
  • 0

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com