Anti-RFC1 抗体 (ab3853)
Key features and details
- Rabbit polyclonal to RFC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RFC1 antibody
RFC1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RFC1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chimpanzee, Gorilla -
免疫原
Synthetic peptide within Human RFC1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P35251 -
ポジティブ・コントロール
- WB: HeLa and HEK-293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3853の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 128 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 128 kDa. |
ターゲット情報
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機能
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. -
組織特異性
Wide tissue distribution. Undetectable in placental tissue. -
配列類似性
Belongs to the activator 1 large subunit family.
Contains 1 BRCT domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5981 Human
- Entrez Gene: 19687 Mouse
- Omim: 102579 Human
- SwissProt: P35251 Human
- SwissProt: P35601 Mouse
- Unigene: 507475 Human
- Unigene: 148877 Mouse
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別名
- A1 140 kDa subunit antibody
- A1 antibody
- A1 P145 Activator 1 large subunit antibody
see all
画像
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All lanes : Anti-RFC1 antibody (ab3853) at 0.4 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 128 kDa
Exposure time: 3 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab3853 は 4 報の論文で使用されています。
- Wessel SR et al. Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators. Cell Rep 28:3497-3509.e4 (2019). PubMed: 31553917
- Castaño E et al. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLoS One 12:e0170389 (2017). WB . PubMed: 28103309
- Lee KY et al. ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin. J Cell Biol 200:31-44 (2013). WB ; Human . PubMed: 23277426
- Lee KY et al. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem 285:10362-9 (2010). PubMed: 20147293