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Anti-Rex1 抗体 [5E11E7] (ab175431)

Submit a review Submit a question References (1)
Western blot - Anti-Rex1 antibody [5E11E7] (ab175431)
  • Western blot - Anti-Rex1 antibody [5E11E7] (ab175431)
  • Western blot - Anti-Rex1 antibody [5E11E7] (ab175431)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (ab175431)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (ab175431)
  • Flow Cytometry - Anti-Rex1 antibody [5E11E7] (ab175431)

Key features and details

  • Mouse monoclonal [5E11E7] to Rex1
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Rex1 antibody [5E11E7]
    Rex1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [5E11E7] to Rex1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, WB, Flow Cytmore details

  • 種交差性

    交差種: Human, Recombinant fragment

  • 免疫原

    Recombinant fragment corresponding to Human Rex1 aa 249-310. (Expressed in E.coli).
    Sequence:

    FEGCGKRFSLDFNLRTHVRIHTGEKRFVCPFQGCNRRFIQSNNLKAHILT HANTNKNEQEGK


    Database link: Q96MM3
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Rex 1 recombinant protein; Rex1 (aa249-310) - hIgGFc transfected HEK293 cell lysate; Jurkat, Raji, HEK293 and PC-3 cell lysates; HEK293 cells; Human rectum cancer tissue; Human esophagus cancer tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    Contains 0.5% protein stabiliser.
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Purified from tissue culture supernatant.

  • ポリ/モノ

    モノクローナル

  • クローン名

    5E11E7

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab175431の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/200 - 1/1000.
WB
1/500 - 1/2000. Predicted molecular weight: 35 kDa.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
IHC-P
1/200 - 1/1000.
WB
1/500 - 1/2000. Predicted molecular weight: 35 kDa.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Involved in self-renewal property of ES cells (By similarity). May be involved in transcriptional regulation.

  • 組織特異性

    Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.

  • 配列類似性

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • hREX-1 antibody
      • Reduced Expression 1 Protein antibody
      • Reduced expression protein 1 antibody
      • Rex-1 antibody
      • REX1 antibody
      • REX1 transcription factor antibody
      • Zfp-42 antibody
      • Zfp42 antibody
      • ZFP42 zinc finger protein antibody
      • ZFP42_HUMAN antibody
      • Zinc finger protein 42 homolog antibody
      • Zinc finger protein 754 antibody
      • ZNF754 antibody
      see all

    画像

    • Western blot - Anti-Rex1 antibody [5E11E7] (ab175431)
      Western blot - Anti-Rex1 antibody [5E11E7] (ab175431)
      All lanes : Anti-Rex1 antibody [5E11E7] (ab175431) at 1/500 dilution

      Lane 1 : HEK293 cell lysate
      Lane 2 : Rex1 (aa 249-310) - hIgGFc transfected HEK293 cell lysate

      Predicted band size: 35 kDa

    • Western blot - Anti-Rex1 antibody [5E11E7] (ab175431)
      Western blot - Anti-Rex1 antibody [5E11E7] (ab175431)
      Anti-Rex1 antibody [5E11E7] (ab175431) at 1/500 dilution + Rex1 recombinant protein

      Predicted band size: 35 kDa



      Expected MWt is 32.7 kDa.

    • Western blot - Anti-Rex1 antibody [5E11E7] (ab175431)
      Western blot - Anti-Rex1 antibody [5E11E7] (ab175431)
      All lanes : Anti-Rex1 antibody [5E11E7] (ab175431) at 1/500 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HEK293 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : PC-3 cell lysate

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (ab175431)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (ab175431)

      Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue labeling Rex1 with ab175431 at 1/200 dilution with DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (ab175431)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rex1 antibody [5E11E7] (ab175431)

      Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Rex1 with ab175431 at 1/200 dilution with DAB staining.

    • Flow Cytometry - Anti-Rex1 antibody [5E11E7] (ab175431)
      Flow Cytometry - Anti-Rex1 antibody [5E11E7] (ab175431)

      Flow cytometric analysis of HEK293 cells labeling Rex1 with ab175431 at 1/200 dilution (green) compared to a negative control (red).

    参考文献 (1)

    ab175431 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab175431 は 1 報の論文で使用されています。

    • Naeem A  et al. Expansion of human amniotic epithelial cells using condition cell reprogramming technology. Hum Cell 36:602-611 (2023). PubMed: 36586053

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