Anti-Retinoid X Receptor alpha/RXRA 抗体 (ab24363)
Key features and details
- Goat polyclonal to Retinoid X Receptor alpha/RXRA
- Suitable for: Flow Cyt (Intra), WB, ICC
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Retinoid X Receptor alpha/RXRA antibody
Retinoid X Receptor alpha/RXRA 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Retinoid X Receptor alpha/RXRA -
由来種
Goat -
特異性
This does not cross-react with either RXR beta or gamma. -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICCmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Retinoid X Receptor alpha/RXRA aa 14-28 (N terminal).
Sequence:(C)QVNSSLTSPTGRGSM
-
ポジティブ・コントロール
- Flow Cyt (Intra): MCF7 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24363の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
|
WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
1 hour primary incubation is recommended for this product. |
ICC |
Use a concentration of 10 µg/ml.
|
特記事項 |
---|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). 1 hour primary incubation is recommended for this product. |
ICC
Use a concentration of 10 µg/ml. |
ターゲット情報
-
機能
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. -
組織特異性
Highly expressed in liver, also found in lung, kidney and heart. -
配列類似性
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain and a C-terminal ligand-binding domain (AF2 domain). -
翻訳後修飾
Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain. Constiutively phosphorylated on Ser-21 in the presence or absence of ligand. Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.
Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 6256 Human
- Entrez Gene: 20181 Mouse
- Entrez Gene: 25271 Rat
- Omim: 180245 Human
- SwissProt: P19793 Human
- SwissProt: P28700 Mouse
- SwissProt: Q05343 Rat
- Unigene: 590886 Human
see all -
別名
- FLJ00280 antibody
- FLJ00318 antibody
- FLJ16020 antibody
see all
画像
-
All lanes : Anti-Retinoid X Receptor alpha/RXRA antibody (ab24363) at 0.3 µg/ml
Lane 1 : HeLa nuclear lysate (in RIPA buffer)
Lane 2 : HeLa nuclear lysate (in RIPA buffer) with peptide
Lane 3 : K562 nuclear lysate (in RIPA buffer)
Lane 4 : K562 nuclear lysate (in RIPA buffer) with peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 51 kDa
Observed band size: 55-60 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
-
Immunocytochemistry analysis of MCF7 cells labelling Retinoid X Receptor alpha/RXRA with ab24363 at 10 µg/mL showing strong nuclear staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Actin filaments were stained with phalloidin (red). Nuclear DNA was labelled with DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
-
Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) labelling Retinoid X Receptor alpha/RXRA with ab24363. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab24363 は 3 報の論文で使用されています。
- Zhao X et al. Risk-Associated Long Noncoding RNA FOXD3-AS1 Inhibits Neuroblastoma Progression by Repressing PARP1-Mediated Activation of CTCF. Mol Ther 26:755-773 (2018). PubMed: 29398485
- Castella B et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). PubMed: 28580927
- Nickkho-Amiry M et al. Peroxisome proliferator-activated receptors modulate proliferation and angiogenesis in human endometrial carcinoma. Mol Cancer Res 10:441-53 (2012). WB, IHC-P ; Human . PubMed: 22205725