Anti-Retinoic Acid Receptor gamma 1 抗体 (ab5904)
Key features and details
- Rabbit polyclonal to Retinoic Acid Receptor gamma 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Retinoic Acid Receptor gamma 1 antibody -
製品の詳細
Rabbit polyclonal to Retinoic Acid Receptor gamma 1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit -
免疫原
Synthetic peptide corresponding to Retinoic Acid Receptor gamma 1 aa 40-57 (N terminal).
Sequence:EMLSPSFRGLGQPDLPKE
(Peptide available asab39773) -
ポジティブ・コントロール
- WB: MCF7, BeWo, HaCaT, HepG2 and HEK293 cell lysate. Mouse Mammary Gland and Rat brain tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5904の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
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特記事項 |
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WB
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
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機能
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5916 Human
- Entrez Gene: 19411 Mouse
- Entrez Gene: 685072 Rat
- Omim: 180190 Human
- SwissProt: P13631 Human
- SwissProt: P18911 Mouse
- Unigene: 1497 Human
- Unigene: 733399 Human
see all -
別名
- NR1B3 antibody
- Nuclear receptor subfamily 1 group B member 3 antibody
- RAR gamma antibody
see all
画像
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All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/500 dilution
Lane 1 : MCF7 Nuclear enriched extract lysate
Lane 2 : BeWo Nuclear enriched extract lysate
Lane 3 : HaCaT Nuclear enriched extract lysate
Lane 4 : HepG2 Nuclear enriched extract lysate
Lane 5 : Mouse Mammary Gland tissue lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Predicted band size: 54 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Detection: chemiluminescence.
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All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
Lane 1 : HEK293 cell lysate with neutralizing peptide
Lane 2 : HEK293 cell lysate
Predicted band size: 54 kDa -
Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/250 dilution + Rat brain tissue lysate at 30 µg
Secondary
Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/ml
Predicted band size: 54 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Detection: chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab5904 は 2 報の論文で使用されています。
- Kadigamuwa C et al. Role of Retinoic Acid Receptor-? in DNA Damage-Induced Necroptosis. iScience 17:74-86 (2019). PubMed: 31255985
- Joss-Moore L et al. Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups. Pediatr Res 73:612-20 (2013). PubMed: 23419538