Anti-Retinoic Acid Receptor beta 抗体

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody (AB5792)

Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate cells on 70% confluent log phase labeling Retinoic Acid Receptor beta. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ab5792 at 1/100 dilution in 0.1% BSA and incubated overnight at 4°C and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e represents BeWo (human placenta choriocarcinoma cell line) having no expression of Retinoic Acid Receptor beta. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-Retinoic Acid Receptor beta antibody (AB5792)

Detection : chemiluminescence

Western blot demonstrating antibody specificity by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. The expression was observed in SH-SY5Y and HEK-293 and not seen in PANC-1, OVCAR-3 and BeWo using ab5792.

All lanes:

Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792) at 1 µg/mL

Lane 1:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 30 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 30 µg

Lane 3:

PANC-1 (Human pancreatic epithelial cancinoma cell line) whole cell lysate at 30 µg

Lane 4:

OVCAR-3 (Human ovary adenocarcinoma cell line) whole cell lysate at 30 µg

Lane 5:

BeWo (human placenta choriocarcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

Predicted band size: 50 kDa

Observed band size: ~58.50 kDa

false

• WB

Supplier Data

Western blot - Anti-Retinoic Acid Receptor beta antibody (AB5792)

All lanes:

Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 25 µg

Lane 2:

HepG2 cell lysate at 25 µg

Lane 3:

Mouse heart cell lysate at 25 µg

Predicted band size: 50 kDa

Observed band size: 58 kDa

false