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Anti-Reticulon 1A 抗体 [MON160] (ab8957)

Submit a review Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)
  • Schematic Diagram - Anti-Reticulon 1A antibody [MON160] (ab8957)

Key features and details

  • Mouse monoclonal [MON160] to Reticulon 1A
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Reticulon 1A antibody [MON160]
    Reticulon 1A 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MON160] to Reticulon 1A

  • 由来種

    Mouse

  • 特異性

    This antibody exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment within Reticulon 1A aa 1-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).
    Database link: Q16799

  • エピトープ

    This antibody is reactive with residues 174-337 of Reticulon-1A.

  • 特記事項

    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 一次抗体 備考

    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MON160

  • ミエローマ

    unknown

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8957の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.

Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
特記事項
IHC-P
Use at an assay dependent concentration.

Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

ターゲット情報

  • 関連性

    Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing.

  • 細胞内局在

    Endoplasmic reticulum

  • 参照データベース

    • 別名

      • Neuroendocrine specific protein antibody
      • NSP antibody
      • Reticulon1 antibody
      • RTN1 antibody

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)

      NSP-A immunostaining in human cerebellum using ab8957.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Reticulon 1A antibody [MON160] (ab8957)

      Human carcinoid (A) and small cell lung carcinoma (B) in paraffin sections, immunostained for NSP-A with ab8957.

    • Schematic Diagram - Anti-Reticulon 1A antibody [MON160] (ab8957)
      Schematic Diagram - Anti-Reticulon 1A antibody [MON160] (ab8957)

      Schematic representation of of the deduced domain structure of the three NSP-gene encoded proteins, NSP-A, NSP-B and NSP-C and mapping of the epitopes recognized by the different NSP monoclonal antibodies.

    参考文献 (3)

    ab8957 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8957 は 3 報の論文で使用されています。

    • Fan Y  et al. Inhibition of Reticulon-1A-Mediated Endoplasmic Reticulum Stress in Early AKI Attenuates Renal Fibrosis Development. J Am Soc Nephrol 28:2007-2021 (2017). PubMed: 28137829
    • Xiao W  et al. Knockdown of RTN1A attenuates ER stress and kidney injury in albumin overload-induced nephropathy. Am J Physiol Renal Physiol 310:F409-15 (2016). PubMed: 26739891
    • Fan Y  et al. RTN1 mediates progression of kidney disease by inducing ER stress. Nat Commun 6:7841 (2015). PubMed: 26227493

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