Anti-Reptin/TIP49B/RUVB2 抗体 (ab36569)
Key features and details
- Rabbit polyclonal to Reptin/TIP49B/RUVB2
- Suitable for: WB, ICC/IF
- Reacts with: Human, Zebrafish
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Reptin/TIP49B/RUVB2 antibody
Reptin/TIP49B/RUVB2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Reptin/TIP49B/RUVB2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human, Zebrafish
交差が予測される動物種: Mouse, Rat, Cow, Xenopus laevis -
免疫原
Synthetic peptide corresponding to Human Reptin/TIP49B/RUVB2 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab36568) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36569の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (8) |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. -
組織特異性
Ubiquitously expressed. Highly expressed in testis and thymus. -
配列類似性
Belongs to the ruvB family. -
ドメイン
The C-terminal domain is required for association with ATF2. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 511048 Cow
- Entrez Gene: 10856 Human
- Entrez Gene: 20174 Mouse
- Entrez Gene: 292907 Rat
- Entrez Gene: 380092 Xenopus laevis
- Entrez Gene: 317678 Zebrafish
- Omim: 604788 Human
- SwissProt: Q2TBU9 Cow
see all -
別名
- 48 kDa TATA box-binding protein-interacting protein antibody
- 48 kDa TBP-interacting protein antibody
- 48-kDa TATA box-binding protein-interacting protein antibody
see all
画像
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All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : A-431 whole cell lysate (ab7909)
Lane 4 : HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 8 : U2OS Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted? -
ICC/IF image of ab36569 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab36569, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Western blot - Anti-Reptin/TIP49B/RUVB2 antibody (ab36569)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/ml
Lane 1 : Hela cells treated with reptin-specific siRNA#1, whole cell lysate
Lane 2 : Hela cells treated with reptin-specific siRNA#2, whole cell lysate
Lane 3 : Hela cells treated with non-specific siRNA, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor® 680 conjugated goat anti-rabbit
at 1/7000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Detection method was Odyssey, with 10 second exposure. -
All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab36569) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab36569 は 17 報の論文で使用されています。
- Cheung CY et al. Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2. J Cell Sci 135:N/A (2022). PubMed: 35635291
- Yenerall P et al. RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer. Cell Chem Biol 27:105-121.e14 (2020). PubMed: 31883965
- Da Ros M et al. FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy 13:302-321 (2017). PubMed: 27929729
- Cloutier P et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun 8:15615 (2017). WB . PubMed: 28561026
- Milone MR et al. Proteomic characterization of peroxisome proliferator-activated receptor-? (PPAR?) overexpressing or silenced colorectal cancer cells unveils a novel protein network associated with an aggressive phenotype. Mol Oncol 10:1344-62 (2016). PubMed: 27499265