Anti-RENT1/hUPF1 抗体 (ab10510)
Key features and details
- Goat polyclonal to RENT1/hUPF1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RENT1/hUPF1 antibody
RENT1/hUPF1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RENT1/hUPF1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide corresponding to Human RENT1/hUPF1 aa 250-350. NP_002902.2 (GeneID 5976)
Database link: Q92900 -
ポジティブ・コントロール
- WB: Whole cell lysate from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).; HeLa, NIH/3T3 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10510の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000 - 1/2500. Detects a band of approximately 140 kDa (predicted molecular weight: 134 kDa).
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特記事項 |
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WB
1/1000 - 1/2500. Detects a band of approximately 140 kDa (predicted molecular weight: 134 kDa). |
ターゲット情報
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機能
Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the DNA2/NAM7 helicase family.
Contains 1 C2H2-type zinc finger. -
ドメイン
The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family. -
翻訳後修飾
Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5976 Human
- Entrez Gene: 19704 Mouse
- Entrez Gene: 406783 Zebrafish
- Omim: 601430 Human
- SwissProt: Q92900 Human
- SwissProt: Q9EPU0 Mouse
- Unigene: 515266 Human
- Unigene: 390048 Mouse
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別名
- ATP dependent helicase RENT1 antibody
- ATP-dependent helicase RENT1 antibody
- Delta helicase antibody
see all
画像
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All lanes : Anti-RENT1/hUPF1 antibody (ab10510) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Predicted band size: 134 kDa
Exposure time: 30 seconds -
ab10510 detection of human RENT1 by Western blot. Samples: Whole cell lysate (30
µ g) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: ab10510 used at 1µ g/ml. Detection: Chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab10510 は 3 報の論文で使用されています。
- Truong DM et al. Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation. PLoS Genet 11:e1005422 (2015). PubMed: 26241656
- Henderson-Smith A et al. SMG1 identified as a regulator of Parkinson's disease-associated alpha-synuclein through siRNA screening. PLoS One 8:e77711 (2013). WB . PubMed: 24204929
- Shi J et al. Caffeine regulates alternative splicing in a subset of cancer-associated genes: a role for SC35. Mol Cell Biol 28:883-95 (2008). WB ; Human . PubMed: 18025108