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リコンビナントRabMAb

Anti-Renilla Luciferase 抗体 [EPR17791] (ab185925)

Reviews (1) Submit a question References (7)
Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
  • Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
  • Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17791] to Renilla Luciferase
  • Suitable for: WB, ICC/IF

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

製品の概要

  • 製品名

    Anti-Renilla Luciferase antibody [EPR17791]
    Renilla Luciferase 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17791] to Renilla Luciferase

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Renilla reniformis
    非交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Renilla Luciferase transfected HEK293T whole cell lysate. ICC/IF: Renilla Luciferase transfected HEK293T cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR17791

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185925の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF
1/1000.
特記事項
WB
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ICC/IF
1/1000.

ターゲット情報

  • 関連性

    The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.

  • 別名

    • Renilla luciferin 2 monooxygenase antibody
    • Renilla type luciferase antibody

画像

  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

    Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
    Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa


    Exposure time: 15 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    Immunocytochemistry/ Immunofluorescence - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Renilla Luciferase, labeling Renilla Luciferase with ab185925 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK-293T cells transfected with Renilla Luciferase is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab185925 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

    Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
    Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) lysate
    Lane 5 : Jurkat (Human T cell leukemia cells from peripheral blood) lysate
    Lane 6 : SH-SY5Y (Human neuroblastoma from bone marrow cells) lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

    Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate at 20 µg
    Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg
    Lane 3 : Human fetal brain lysate at 10 µg
    Lane 4 : Human fetal heart lysate at 10 µg
    Lane 5 : Human fetal kidney lysate at 10 µg
    Lane 6 : Human fetal spleen lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    Western blot - Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    All lanes : Anti-Renilla Luciferase antibody [EPR17791] (ab185925) at 1/1000 dilution

    Lane 1 : Empty vector (vector control) transfected HEK293 (Human embryonic kidney) whole cell lysate
    Lane 2 : Renilla Luciferase transfected HEK293T (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse heart lysate
    Lane 5 : Mouse kidney lysate
    Lane 6 : Mouse spleen lysate
    Lane 7 : Rat brain lysate
    Lane 8 : Rat heart lysate
    Lane 9 : Rat kidney lysate
    Lane 10 : Rat spleen lysate
    Lane 11 : C6 (Rat glial tumor cells) lysate
    Lane 12 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
    Lane 13 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
    Lane 14 : NIH/3T3 (Mouse embyro fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Renilla Luciferase antibody [EPR17791] (ab185925)
    Anti-Renilla Luciferase antibody [EPR17791] (ab185925)

参考文献 (7)

ab185925 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185925 は 7 報の論文で使用されています。

  • Paudel DB & Sanfaçon H Mapping of sequences in the 5' region and 3' UTR of tomato ringspot virus RNA2 that facilitate cap-independent translation of reporter transcripts in vitro. PLoS One 16:e0249928 (2021). PubMed: 33836032
  • Weber R  et al. 4EHP and GIGYF1/2 Mediate Translation-Coupled Messenger RNA Decay. Cell Rep 33:108262 (2020). PubMed: 33053355
  • Niu J  et al. Non-invasive bioluminescence imaging of HCoV-OC43 infection and therapy in the central nervous system of live mice. Antiviral Res 173:104646 (2020). PubMed: 31705922
  • Scholz A  et al. Translation of TNFAIP2 is tightly controlled by upstream open reading frames. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31392347
  • Zhou Y  et al. Disruption of TWIST1 translation by 5' UTR variants in Saethre-Chotzen syndrome. Hum Mutat 39:1360-1365 (2018). PubMed: 30040876
  • Braid LR  et al. Intramuscular administration potentiates extended dwell time of mesenchymal stromal cells compared to other routes. Cytotherapy 20:232-244 (2018). PubMed: 29167063
  • Shen L  et al. Safe and Sensitive Antiviral Screening Platform Based on Recombinant Human Coronavirus OC43 Expressing the Luciferase Reporter Gene. Antimicrob Agents Chemother 60:5492-503 (2016). PubMed: 27381385

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Western blot abreview for Anti-Renilla Luciferase antibody [EPR17791]

 Good
Abreviews
Application
Western blot
Sample
Renilla Luciferase Cell lysate - whole cell (Pancreas)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
Pancreas
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Sep 30 2022

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