AB255820

Anti-REG4 抗体 [EPR22810-327]

Anti-REG4 antibody [EPR22810-327]

BOND RX™ Validated
RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal REG4 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 7 publications.

別名を表示する

GISP, RELP, REG4, Regenerating islet-derived protein 4, REG-4, Gastrointestinal secretory protein, REG-like protein, Regenerating islet-derived protein IV, Reg IV

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (AB255820)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (AB255820)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling REG4 with ab255820 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon (PMID : 25295732) is observed. The section was incubated with ab255820 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (AB255820)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling REG4 with ab255820 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon carcinoma (PMID : 25295732) is observed. The section was incubated with ab255820 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Unknown

Immunoprecipitation - Anti-REG4 antibody [EPR22810-327] (AB255820)

REG4 was immunoprecipitated from 0.35 mg LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10μg with ab255820 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255820 1/1000 dilution (0.52 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10μg Lane 2 : ab255820 IP in LoVo whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab255820 in LoVo whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 min

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Immunoprecipitation - Anti-REG4 antibody [EPR22810-327] (ab255820)

Predicted band size: 18 kDa

false

Unknown

Western blot - Anti-REG4 antibody [EPR22810-327] (AB255820)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 20126989).

Negative control : HT-29 (PMID : 12455032).

Blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-REG4 antibody [EPR22810-327] (ab255820) at 1/1000 dilution

All lanes:

LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 14 kDa

false

Exposure time: 59s

Unknown

Western blot - Anti-REG4 antibody [EPR22810-327] (AB255820)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-REG4 antibody [EPR22810-327] (ab255820) at 1/1000 dilution

All lanes:

Recombinant Human REG4 Protein at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 14 kDa

false

Exposure time: 59s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22810-327

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

REG4 also known as Reg IV or regenerating islet-derived 4 is a protein with a mass of about 19 kDa. It belongs to the regenerating gene family and has roles in cell growth and regeneration. REG4 expression occurs mainly in the gastric antrum small intestine colon and pancreas. It also appears in certain epithelial tissues particularly ones undergoing regeneration or transformation. REG4's structure includes a C-type lectin domain which suggests its potential involvement in cell adhesion or signaling.

Biological function summary

REG4 strengthens the protection of epithelial cells and supports regeneration during tissue damage or inflammation. It acts as part of cell growth and survival pathways and does not form large complexes on its own. The protein can promote proliferation and maintain integrity in epithelial tissues influencing both normal and cancerous cell behavior. The presence of REG4 might modulate cell response to environmental stress and impact cellular differentiation processes.

Pathways

REG4 impacts both the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are important for cell proliferation and survival indicating REG4's involvement in cellular stress response and oncogenesis. REG4 interacts with other proteins such as EGFR (Epidermal Growth Factor Receptor) which participate in these pathways. This protein co-activity may enhance cellular responses contributing to growth regulation and survival signaling in various tissues.

REG4 has a strong association with colorectal cancer and inflammatory bowel disease (IBD). Its overexpression has been noted in colorectal cancer tissues and can relate to tumor growth and metastasis. In the context of IBD REG4 expression connects with inflammation markers and mucosal healing processes. Proteins like MUC2 (Mucin 2) and CDX2 (Caudal Type Homeobox 2) involved in these diseases show relationships with REG4 emphasizing its role in disease progression and regulation within epithelial tissues.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium.

See full target information REG4

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:573 PubMed40188179

2025

Targeting alveolar epithelial cells with lipid micelle-encapsulated necroptosis inhibitors to alleviate acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Ying Kang,Nan-Xia Xuan,Qi-Chao Zhou,Qian-Yu Huang,Meng-Jia Yu,Gen-Sheng Zhang,Wei Cui,Zhao-Cai Zhang,Yang Du,Bao-Ping Tian

British journal of cancer 131:271-282 PubMed38862740

2024

Tumour cell-derived serglycin promotes IL-8 secretion of CAFs in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Li,Guiping Xie,Jia Chen,Yaohui Wang,Jing Zhai,Lizong Shen

PeerJ 12:e17350 PubMed38827297

2024

Single-cell transcriptome reveals the heterogeneity of malignant ductal cells and the prognostic value of REG4 and SPINK1 in primary pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yutian Ji,Qianhui Xu,Weilin Wang

iScience 26:107829 PubMed37736049

2023

Colitis-induced upregulation of tumor necrosis factor receptor-2 (TNFR2) terminates epithelial regenerative signaling to restore homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zohreh Sharifkhodaei,Cambrian Y Liu,Nandini Girish,Ying Huang,Shivesh Punit,M Kay Washington,D Brent Polk

Cell host & microbe 31:1450-1468.e8 PubMed37652008

2023

Antimicrobial overproduction sustains intestinal inflammation by inhibiting Enterococcus colonization.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Ku Jang,Thomas Heaney,Mariya London,Yi Ding,Gregory Putzel,Frank Yeung,Defne Ercelen,Ying-Han Chen,Jordan Axelrad,Sakteesh Gurunathan,Chaoting Zhou,Magdalena Podkowik,Natalia Arguelles,Anusha Srivastava,Bo Shopsin,Victor J Torres,A Marijke Keestra-Gounder,Alejandro Pironti,Matthew E Griffin,Howard C Hang,Ken Cadwell

Communications biology 6:85 PubMed36690709

2023

Single-cell profiling reveals differences between human classical adenocarcinoma and mucinous adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Jie Hu,Ying-Jie Li,Li Zhang,Deng-Bo Ji,Xin-Zhi Liu,Yong-Jiu Chen,Lin Wang,Ai-Wen Wu

OncoTargets and therapy 14:1605-1611 PubMed33688207

2021

is a Potential Biomarker for Radiochemotherapy Sensitivity in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Gao,Xingjun Wu,Libo Zhang,Yang Dai,Zhe Zhu,Yunqing Zhi,Kaijing Wang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-REG4 抗体 [EPR22810-327]

Anti-REG4 antibody [EPR22810-327]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (AB255820)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG4 antibody [EPR22810-327] (AB255820)

Immunoprecipitation - Anti-REG4 antibody [EPR22810-327] (AB255820)

Western blot - Anti-REG4 antibody [EPR22810-327] (AB255820)

Western blot - Anti-REG4 antibody [EPR22810-327] (AB255820)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (7)

Abcam product promise