Anti-REG1 alpha 抗体 (ab47099)
Key features and details
- Rabbit polyclonal to REG1 alpha
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-REG1 alpha antibody
REG1 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to REG1 alpha -
由来種
Rabbit -
特異性
This antibody is specific for REG 1 alpha. It was tested in a sandwich ELISA with antigens: Reg 1beta, PAP=(REG III), and Reg IV. We did not detect any crossreactivity. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant human REG 1 alpha. A 17.8 kDa protein containing 144 amino acid residues of the human REG 1 alpha and 12 additional amino acid residues, HisTag. Source of immunogen E.coli
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ポジティブ･コントロール
- recombinant protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.134% PBS, 0.58% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Immunoaffinity chromatography on a column with immobilized recombinant human REG 1 alpha. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47099の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).
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| 特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa). |
ターゲット情報
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機能
Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. -
組織特異性
In pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients. -
配列類似性
Contains 1 C-type lectin domain. -
発生段階
High expression levels in fetal and infant brains; much lower in adult brains. -
翻訳後修飾
The composition of the O-linked carbohydrate on Thr-27 is complex and varied. In the crystallographic structure, the attached sugar appears to be N-acetylglucosamine, typical of an intracellular protein, rather than N-acetylgalactosamine. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5967 Human
- Omim: 167770 Human
- SwissProt: P05451 Human
- Unigene: 4158 Human
- Unigene: 49407 Human
- Unigene: 708865 Human
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別名
- ICRF antibody
- Islet cells regeneration factor antibody
- Islet of Langerhans regenerating protein antibody
see all
画像
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Western blot - Anti-REG1 alpha antibody (ab47099)Anti-REG1 alpha antibody (ab47099) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 30 seconds
The band observed at 14 kDa could potentially be a cleaved form of REG1 alpha due to the presence of a 25 amino acid signal peptide.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab47099 は 5 報の論文で使用されています。
- Lebart MC et al. Reg-1α, a New Substrate of Calpain-2 Depending on Its Glycosylation Status. Int J Mol Sci 23:N/A (2022). PubMed: 35955718
- Su C et al. Single-Cell RNA Sequencing in Multiple Pathologic Types of Renal Cell Carcinoma Revealed Novel Potential Tumor-Specific Markers. Front Oncol 11:719564 (2021). PubMed: 34722263
- Saitoh S et al. Improved Serial Sectioning Techniques for Correlative Light-Electron Microscopy Mapping of Human Langerhans Islets. Acta Histochem Cytochem 51:9-20 (2018). PubMed: 29622846
- Ferretti E et al. Impaired antimicrobial response and mucosal protection induced by ibuprofen in the immature human intestine. Pediatr Res 84:813-820 (2018). PubMed: 30341414
- Radon TP et al. Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma. Clin Cancer Res 21:3512-21 (2015). PubMed: 26240291
