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AB242010

redFluor™ 710 Anti-CD4 抗体 [GK1.5]

redFluor™ 710 Anti-CD4 antibody [GK1.5]

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Anti-CD4 antibody [GK1.5] conjugated to redFluor™ 710 - suitable for flow cytometry, and reacts with mouse samples. Clone [GK1.5] is a rat monoclonal antibody and one of the most commonly used clones for flow cytometry.

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別名を表示する

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

1 Images
Flow Cytometry - redFluor™ 710 Anti-CD4 antibody [GK1.5] (AB242010)
  • Flow Cyt

Supplier Data

Flow Cytometry - redFluor™ 710 Anti-CD4 antibody [GK1.5] (AB242010)

Flow cytometric analysis of C57Bl/6 splenocytes labeling CD4 with 0.06 μg ab242010 (solid line) or 0.06 μg redFluor™ 710 Rat IgG2b (dashed line).

関連する標識済み抗体及び組成の異なる製品 (6)

  • 660 APC

    APC Anti-CD4 antibody [GK1.5]

  • 519 FITC

    FITC Anti-CD4 antibody [GK1.5]

  • 519 FITC

    FITC Anti-CD4 antibody [GK1.5]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD4 antibody [GK1.5]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD4 antibody [GK1.5]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD4 antibody [GK1.5]

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

GK1.5

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

標識

redFluor™ 710

励起波長/蛍光波長

Ex: 684nm, Em: 704nm

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt

applications

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 0.06 μg. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.</p>" } } }

製品の詳細

If used together, the GK1.5 antibody and an alternative antibody, anti-Mouse CD4 clone RM4-5, will "compete" for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
Purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
バッファー組成
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Store in the dark

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
See full target information Cd4

Abcam product promise

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