Anti-RED1 抗体 [EPR25433-101] - BSA and Azide free (ab290649)
![Western blot - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-484199-WB1.jpg "Western blot - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25433-101] to RED1 - BSA and Azide free
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RED1 antibody [EPR25433-101] - BSA and Azide free
RED1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25433-101] to RED1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-P, Flow Cyt (Intra)more details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa whole cell lysate, HEL whole cell lysate, NIH/3T3 whole cell lysate, C2C12 whole cell lysate, human placenta tissues lysates, human kidney tissues lysates, mouse brain tissue lysates, rat brain tissue lysates, rat placenta tissue lysate, HEK-293 transfected with ADARB1(WT) expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: human cerebrum and kidney, mouse and rat cerebrum. ICC/IF: HeLa, NIH/3T3. Flow cyt. Intr.: HeLa, NIH/3T3.
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特記事項
ab290649 is a carrier free version of ab290638.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25433-101 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab290649の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 81 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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機能
Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis. -
組織特異性
Highly expressed in brain and heart and at lower levels in placenta. Fair expression in lung, liver and kidney. Detected in brain, heart, kidney, lung and liver (at protein level). Isoform 5 is high expressed in hippocampus and colon. Isoform 5 is expressed in pediatric astrocytomas and the protein has a decreased RNA-editing activity. The decrease in RNA editing correlates with the grade of malignancy of the tumors, with the high grade tumors showing lower editing is seen. -
配列類似性
Contains 1 A to I editase domain.
Contains 2 DRBM (double-stranded RNA-binding) domains. -
細胞内局在
Nucleus. Nucleus > nucleolus. Shuttles between nucleoli and the nucleoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 104 Human
- Entrez Gene: 110532 Mouse
- Entrez Gene: 25367 Rat
- Omim: 601218 Human
- SwissProt: P78563 Human
- SwissProt: Q91ZS8 Mouse
- SwissProt: P51400 Rat
- Unigene: 474018 Human
see all -
別名
- ADARB 1 antibody
- ADARB1 antibody
- 1700057H01Rik antibody
see all
画像
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Western blot - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)All lanes : Anti-RED1 antibody [EPR25433-101] (ab290638) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEL (human erythroleukemia erythroblast) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : C2C12 ( mouse myoblasts myoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 81 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsThis data was developed using ab290638, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration was 5% NFDM/TBST.
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![Western blot - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-484243-WB2.jpg)
Western blot - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)All lanes : Anti-RED1 antibody [EPR25433-101] (ab290638) at 1/1000 dilution
Lane 2 : Human kidney tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat placenta tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : oat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Lanes 3-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 81 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsThis data was developed using ab290638, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration was 5% NFDM/TBST.
The bolts of lanes 1&2 and lanes 3-5 were incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L, 1:2000) and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051, 1:100, 000), respectively.
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![Western blot - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-484245-WB3.jpg)
Western blot - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)All lanes : Anti-RED1 antibody [EPR25433-101] (ab290638) at 1/1000 dilution
Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293 transfected with ADARB1(WT) expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : HEK-293 transfected with ADARB2 (WT) expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 81 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsThis data was developed using ab290638, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration was 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-3-anti-red1-antibody-epr25433101-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)This data was developed using ab290638, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling RED1 with ab290638 at 1/500 (1.206 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Nuclear staining on human cerebrum. The section was incubated with ab290638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-4-anti-red1-antibody-epr25433101-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)This data was developed using ab290638, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RED1 with ab290638 at 1/500 (1.206 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Nuclear staining on human kidney. The section was incubated with ab290638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-5-anti-red1-antibody-epr25433101-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)This data was developed using ab290638, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling RED1 with ab290638 at 1/500 (1.206 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) . Nuclear staining on mouse cerebrum. The section was incubated with ab290638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-6-anti-red1-antibody-epr25433101-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling RED1 with ab290638 at 1/500 (1.206 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ C Polymer Refine Detection) . Nuclear staining on rat cerebrum. The section was incubated with ab290638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) .
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunocytochemistry/ Immunofluorescence - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-1-anti-red1-antibody-epr25433101-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)This data was developed using ab290638, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling RED1 with ab290638 at 1/50 (12.06 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2µg/mL) dilution (Green). Confocal image showing nuclear staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2µg/mL) dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-2-anti-red1-antibody-epr25433101-immunocytochemistry-nih3t3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)This data was developed using ab290638, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast adherent) cells labelling RED1 with ab290638 at 1/50 (12.06 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2µg/mL) dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2µg/mL) dilution.
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![Flow Cytometry (Intracellular) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-7-anti-red1-antibody-epr25433101-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)This data was developed using ab290638, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling RED1 with ab290638 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-8-anti-red1-antibody-epr25433101-flow-cytometry-nih3t3.jpg)
Flow Cytometry (Intracellular) - Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)This data was developed using ab290638, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labelling RED1 with ab290638 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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![Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)](https://www.abcam.co.jp/ps/products/290/ab290649/Images/ab290649-484197-Benefits-of-Recombinant-antibodies.jpg)
Anti-RED1 antibody [EPR25433-101] - BSA and Azide free (ab290649)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab290649 は論文での使用が確認できていません。
