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Anti-Red Blood Cells 抗体 (ab34858)

Submit a review Q&A (2)References (6)

Key features and details

  • Rabbit polyclonal to Red Blood Cells
  • Suitable for: Agglutination
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Red Blood Cells antibody
    Red Blood Cells 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Red Blood Cells

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Agglutinationmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue/ cell preparation: Human washed pooled Red Blood Cells.

  • 特記事項

    ab34858 is whole antiserum. For your information, we provide you the total protein concentration which is 75-85 mg/ml. Since the antibody is unpurified, we can only estimate its concentration at 1–10 mg/ml.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...

  • 特記事項（精製）

    This product was prepared from polyspecific antiserum by a delipidation and defibrination.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab34858の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Agglutination
Use at an assay dependent concentration.

An agglutination titer against serial dilutions of washed pooled cells on a titer plate of 1:64 was observed for this product.
特記事項
Agglutination
Use at an assay dependent concentration.

An agglutination titer against serial dilutions of washed pooled cells on a titer plate of 1:64 was observed for this product.

ターゲット情報

  • 関連性

    Red blood cells are the most common type of blood cell and are the vertebrate body's principal means of delivering oxygen from the lungs or gills to body tissues via the blood.

  • 別名

    • Erythrocytes antibody
    • RBCs antibody

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (6)

ab34858 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab34858 は 6 報の論文で使用されています。

  • Quintana MDP  et al. Antibodies in children with malaria to PfEMP1, RIFIN and SURFIN expressed at the Plasmodium falciparum parasitized red blood cell surface. Sci Rep 8:3262 (2018). PubMed: 29459776
  • Sun J  et al. Engineered proteins with sensing and activating modules for automated reprogramming of cellular functions. Nat Commun 8:477 (2017). PubMed: 28883531
  • Quintana Mdel P  et al. Phagocytosis-inducing antibodies to Plasmodium falciparum upon immunization with a recombinant PfEMP1 NTS-DBL1a domain. Malar J 15:416 (2016). PubMed: 27531359
  • Ghumra A  et al. Induction of Strain-Transcending Antibodies Against Group A PfEMP1 Surface Antigens from Virulent Malaria Parasites. PLoS Pathog 8:e1002665 (2012). Human . PubMed: 22532802
  • Voisin SN  et al. Identification of Novel Molecular Targets for Endometrial Cancer Using a Drill-Down LC-MS/MS Approach with iTRAQ. PLoS One 6:e16352 (2011). Agglutination ; Human . PubMed: 21305022
  • Ghumra A  et al. Immunisation with Recombinant PfEMP1 Domains Elicits Functional Rosette-Inhibiting and Phagocytosis-Inducing Antibodies to Plasmodium falciparum. PLoS One 6:e16414 (2011). Human . PubMed: 21305024

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Product code: 34858
Inquiry: I am looking for a primary antibody to deplete erythrocytes through immunocapture (attaching the primary antibody to a magnetic particle, then capturing erythrocytes and removing them with a magnetic field). Ab34858 looks encouraging as it agglutinates, demonstrating it sees surface antigens. The 3 references refer to PfEMP1, which is a surface antigen expressed when the malarial parasite infects an RBC - can you confirm that Ab34858 binds to all human erythrocytes before I order and try it. Also if there is any information on immuno-capture, that would be very helpful

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Answer

Thank you for contacting us and your interest in our products.

Unfortunately, I do not have any further information to share in relation to the epitope recognised by the anti-Red Blood Cells antibody (ab34858). We have notourselves performed any detailed studies to elucidate the specific bound antigens.

We have not tested this whole antiserum (ab34858) for the proposed experiment or against all blood types and therefore cannot guarantee the results.This does not mean that it would not work, simply that we have not tested it ourselves.

I am sorry I could not be of more help. If you have any further questions please do not hesitate to ask.

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Question

Would like to try this antibody to stain red blood cells. Would like any additional information on the antibody.

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Answer

Thank you for your call today and for your questions about ab34858. I have checked through my internal notes and the concentration previously listed on the datasheet (90 mg/mL) is incorrect. This antibody is provided as whole antiserum and the concentration is not determined. When staining cells with a whole antiserum, we recommend a 1:50-1:100 dilution.

I've created a discount code which can be redeemed for a free primary antibody after submitting an Abreview for ab34858 in ICC (cell staining). Please see below for more details, and let me know if you have any questions or need anything else.

DISCOUNT CODE: ***
Expiration date: June 16, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab34858 in ICC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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