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Anti-Recoverin 抗体 [6A55CD6] (ab31928)

Reviews (2)Q&A (3)References (4)
Western blot - Anti-Recoverin antibody [6A55CD6] (ab31928)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Recoverin antibody [6A55CD6] (ab31928)

Key features and details

  • Mouse monoclonal [6A55CD6] to Recoverin
  • Suitable for: WB, IHC-FoFr
  • Reacts with: Mouse, Rat
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Recoverin antibody [6A55CD6]

  • 製品の詳細

    Mouse monoclonal [6A55CD6] to Recoverin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-FoFrmore details

  • 種交差性

    交差種: Mouse, Rat
    交差が予測される動物種: Sheep, Cow, Human

  • 免疫原

    Recombinant full length protein corresponding to Human Recoverin.

  • ポジティブ･コントロール

    • ab31928 gave a positive result in Mouse Retina Whole Cell Lysate.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab31928の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
IHC-FoFr (1)
Use at an assay dependent concentration.
特記事項
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
IHC-FoFr
Use at an assay dependent concentration.

ターゲット情報

画像

  • Western blot - Anti-Recoverin antibody [6A55CD6] (ab31928)
    Western blot - Anti-Recoverin antibody [6A55CD6] (ab31928)
    All lanes : Anti-Recoverin antibody [6A55CD6] (ab31928) at 5 µg/ml

    Lane 1 : Mouse Retina Whole Cell Lysate
    Lane 2 : Human Retina Normal Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa


    Exposure time: 8 minutes
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Recoverin antibody [6A55CD6] (ab31928)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Recoverin antibody [6A55CD6] (ab31928)This image is courtesy of an Abreview submitted by Dr Sophie Pezet

    ab31928 staining Recoverin in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in paraformaldehyde without permeation and blocking steps. The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to mouse IgG, diluted 1/1000 was used as secondary.

    See Abreview

参考文献 (4)

ab31928 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab31928 は 4 報の論文で使用されています。

  • Morshedian A  et al. Reproducibility of the Rod Photoreceptor Response Depends Critically on the Concentration of the Phosphodiesterase Effector Enzyme. J Neurosci 42:2180-2189 (2022). PubMed: 35091503
  • Ahamed W  et al. HTRA1 Regulates Subclinical Inflammation and Activates Proangiogenic Response in the Retina and Choroid. Int J Mol Sci 23:N/A (2022). PubMed: 36142120
  • Liu Y  et al. Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells. Int J Mol Med 40:1172-1184 (2017). PubMed: 28848998
  • Redenti S  et al. Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation. Biomaterials 30:3405-14 (2009). ICC/IF ; Mouse . PubMed: 19361860

レビューと Q&A

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1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Recoverin antibody [6A55CD6]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cells from cornea)
Specification
cells from cornea
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 16 2010

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Recoverin antibody [6A55CD6]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sophie Pezet

Verified customer

投稿 Jan 28 2009

Question

Dear Technical Team,
Hope this e-mail finds you well!
Please find below a complaint from one of our customer to whom we supplied
antibodies having cat nos. ab94585, ab11259, ab124025 & ab31928.
The technical forms are also attached with this mail.
Request you to kindly look into it & advise us accordingly to do further
needful on it.
Looking forward towards your valued reply/ comments on it.
Thanking you in advance.
With Best Regards,

Read More
Answer

Thank you for taking the time to complete our questionnaires and contact us. I am sorry to hearthe customer has had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

To confirm with you, four antibodies are mentioned in your email. However, 2 copies of the questionnaire for ab124025 have been provided and there is no questionnaire for ab11259.

I can provide a response for ab94585,ab124025and ab31928. If you require further help regarding ab11529, please do not hesitate to send the questionnaire and I will be pleased to help.

I would like to reassure you that ab94585 and ab31928 are tested and covered by our 6 month guarantee for use in IHC-Fr and mouse samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement. Regarding ab124025, this is tested and guaranteed in mouse, but has not been tested in frozen sections. Could you confirm if the customer has tried paraffin sections, for which we can provide the guarantee?

Reviewing this case, I would like to offer some suggestions to help optimize the results from ab94585,ab124025and ab31928. I would also appreciate if you can confirm some further details. There are similar comments to provide for all three antibodies:

1. Fixation 1 hour for frozen sections is a very long time. For Frozen sections, we recommend fixation for 10 minutes only. Otherwize there may be too much protein crosslinking from the PFA, and the epitope may not be accessible to the antibody.

2. As far as I am aware, Recoverin, GABA A Receptor alpha 1 and Neurokinin 3 Receptor NK3 are all membrane or membraneassociated protein.Detection of thesewill not require permeabilization. Indeed, Triton is a strong detergent and can disrupt membrane proteins and eptiopes, which can significantly affect the staining. Therefore, I can suggest to remove the permeabilization step.

3. I can recommend to use a more gentle detergent, such as Tween 20 in the other buffers, try 0.1% - 0.2% Tween.

4. I recommend to include 0.1 - 0.2% tween in the wash buffer, this will help to wash away any excess antibody.

5. We recommend not to mix blocking agents in an experiment. try serum only.

6. Could you confirm, are the current vials of secondary antibodies working well with other primary antibodies? What were the results of the no primary controls? The concentration of secondary antibody may need to be reduced to help optimize the results.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details, including information regarding ab11259.

Read More

Question

Thank you for your time.  Now to compare suitable antibodies available from several vendors ….

Read More
Answer

It was my pleassure to help you find the information that you needed. When looking at other sources please remember that at Abcam, providing quality service is just as important as providing quality products. Our products are guaranteed under our Abpromise®, which ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund.

The Abcam Abpromise® guarantee:
- 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors.
- We guarantee our products work in the tested species and applications stated on the datasheet.
- We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase.
- We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards.

Please note these conditions to our Abpromise®:
- Protocol information must be provided in order for the claim to be validated.
- Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples.
- “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed.
- Fast Track antibodies are covered for ELISA against the immunizing peptide only.

Please let me know if you have any questions or there are other way in which Abcam might help.

Read More

Question

Thank you for taking the time to explain the similarities of these two products to me. Can you tell me whether ab31928 is purified with Protein A or G to obtain the IgG fraction? Or even affinity purified against the protein to ensure a more pure product?

Read More
Answer

Thank you for contacting us.

This product is Protein G purified.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

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