Recombinant human DYRK3 protein (ab135222)
Key features and details
- Expression system: Escherichia coli
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
製品の詳細
-
製品名
Recombinant human DYRK3 protein -
生理活性
The specific activity of ab135222 was determined to be 242 nmol/min/mg.
-
精製度
> 70 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
MKVEQLFQEFGNRKSNTIQSDGISDSEKCSPTVSQGKSSDCLNTVKSNSS SKAPKVVPLTPEQALKQYKHHLTAYEKLEIINYPEIYFVGPNAKKRHGVI GGPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGSFGQVARVYDHKLR QYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTFRN HVCMAFELLSIDLYELIKKNKFQGFSVQLVRKFAQSILQSLDALHKNKII HCDLKPENILLKHHGRSSTKVIDFGSSCFEYQKLYTYIQSRFYRAPEIIL GSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLACMMELLGMPPPKL LEQSKRAKYFINSKGIPRYCSVTTQADGRVVLVGGRSRRGKKRGPPGSKD WGTALKGCDDYLFIEFLKRCLHWDPSARLTPAQALRHPWISKSVPRPLTT IDKVSGKRVVNPASAFQGLGSKLPPVVGIANKLKANLMSETNGSIPLCSV LPKLIS -
予測される分子量
95 kDa including tags -
領域
63 to 568
-
関連製品
-
Related Products
-
Substrate reagent
特性
Our Abpromise guarantee covers the use of ab135222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
Western blot
-
製品の状態
Liquid -
備考
ab204857 (DYRKtide peptide) can be utilized as a substrate for assessing kinase activity.
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- Dual specificity tyrosine (Y) phosphorylation regulated kinase 3
- Dual specificity tyrosine phosphorylation-regulated kinase 3
- Dual specificity tyrosine-phosphorylation-regulated kinase 3
see all -
機能
Negative regulator of EPO-dependent erythropoiesis, may place an upper limit on red cell production during stress erythropoiesis. Inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. May act by regulating CREB/CRE signaling. -
組織特異性
Isoform 1 and isoform 2 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 is the predominant form in testis. Isoform 1 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on tyrosine residues. -
細胞内局在
Nucleus. - Information by UniProt
画像
-
The specific activity of DYRK3 (ab135222) was determined to be 315 nmol/min/mg as per activity assay protocol and was equivalent to 240 nmol/min/mg as per radiometric assay
-
SDS PAGE analysis of ab135222
-
SDS PAGE analysis of ab135222
-
The specific activity of ab135222 was determined to be 242 nmol/min/mg.
-
SDS-PAGE analysis of ab135222.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab135222 は論文での使用が確認できていません。