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リコンビナントRabMAb

Anti-RCN1/RCN 抗体 [EPR19193] (ab184441)

Submit a review Submit a question References (3)
Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
  • Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
  • Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
  • Anti-RCN1/RCN antibody [EPR19193] (ab184441)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19193] to RCN1/RCN
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RCN1/RCN antibody [EPR19193]
    RCN1/RCN 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19193] to RCN1/RCN

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human fetal liver, heart, kidney and spleen lysates; HeLa, HEK-293, U-87 MG, C6, SH-SY5Y, Neuro-2a, RAW 264.7, PC-12 and NIH/3T3 cell lysates; E14 mouse and rat embryo lysates; Rat cerebral cortex, brain, heart and spleen lysates; Mouse brain, heart and spleen lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR19193

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184441の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000. Detects a band of approximately 44, 46 kDa (predicted molecular weight: 38 kDa).
特記事項
WB
1/2000. Detects a band of approximately 44, 46 kDa (predicted molecular weight: 38 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.

ターゲット情報

画像

  • Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 38 kDa
    Observed band size: 44,46 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2 and 3: 30 seconds; Lane 4: 3 minutes.

  • Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : E14 mouse embryo lysate
    Lane 5 : E14 rat embryo lysate
    Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 7 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 8 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 9 : Rat cerebral cortex lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 44,46 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:  Lane 1-6: 8 seconds; Lane 7 and 8: 30 seconds; Lane 9: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 8416973).

  • Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    Western blot - Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    All lanes : Anti-RCN1/RCN antibody [EPR19193] (ab184441) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat spleen lysate
    Lane 7 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 9 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 38 kDa
    Observed band size: 44,46 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-6: 3 minutes Lane 7 and 8: 30 seconds; Lane 9 and 10: 1 second.

  • Anti-RCN1/RCN antibody [EPR19193] (ab184441)
    Anti-RCN1/RCN antibody [EPR19193] (ab184441)

参考文献 (3)

ab184441 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184441 は 3 報の論文で使用されています。

  • Fu H  et al. Reticulocalbin 1 is required for proliferation and migration of non-small cell lung cancer cells regulated by osteoblast-conditioned medium. J Cell Mol Med 25:11198-11211 (2021). PubMed: 34747128
  • Huang ZH  et al. Reticulocalbin-1 knockdown increases the sensitivity of cells to Adriamycin in nasopharyngeal carcinoma and promotes endoplasmic reticulum stress-induced cell apoptosis. Cell Cycle 19:1576-1589 (2020). PubMed: 32436770
  • Chen X  et al. Overexpression of RCN1 correlates with poor prognosis and progression in non-small cell lung cancer. Hum Pathol 83:140-148 (2019). PubMed: 30172915

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