Anti-RC3H1 抗体 (ab70195)
Key features and details
- Rabbit polyclonal to RC3H1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RC3H1 antibody
RC3H1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RC3H1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to a region between residues 500 and 550 of human RC3H1
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ポジティブ･コントロール
- WB: HeLa and 293T whole cell lysates; TCMK-1, 4T1, CT25.WT IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab70195 was affinity purified using an epitope specific to RC3H1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70195の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
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WB
1/500 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1. -
組織特異性
Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver. -
配列類似性
Contains 1 C3H1-type zinc finger.
Contains 1 RING-type zinc finger. -
細胞内局在
Cytoplasm. Localizes to cytosolic RNA granules, which are sites for regulating mRNA translation and decay, suggesting that it may bind RNA. - Information by UniProt
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参照データベース
- Entrez Gene: 149041 Human
- Entrez Gene: 381305 Mouse
- Omim: 609424 Human
- SwissProt: Q5TC82 Human
- SwissProt: Q4VGL6 Mouse
- Unigene: 30258 Human
- Unigene: 329667 Mouse
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別名
- KIAA2025 antibody
- Rc3h1 antibody
- RC3H1_HUMAN antibody
see all
画像
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Western blot - Anti-RC3H1 antibody (ab70195)All lanes : Anti-RC3H1 antibody (ab70195) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293T whole cell lysate at 50 µg
Predicted band size: 126 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-RC3H1 antibody (ab70195)RC3H1 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70195 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70195 at 0.1 µg/mL.
Lane 1: ab70195 IP in HeLa whole cell lysate.
Lane 2: ab70195 IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
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Western blot - Anti-RC3H1 antibody (ab70195)All lanes : Anti-RC3H1 antibody (ab70195) at 2 µg/ml
Lane 1 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 2 : 4T1 (Mouse mammary gland carcinoma cell line) whole cell lysate
Lane 3 : CT26.WT (Murine Colon Carcinoma) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 126 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab70195 は論文での使用が確認できていません。