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リコンビナントRabMAb

Anti-RBPMS2 抗体 [EPR13121-79] (ab181098)

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Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
  • Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
  • Anti-RBPMS2 antibody [EPR13121-79] (ab181098)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13121-79] to RBPMS2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RBPMS2 antibody [EPR13121-79]
    RBPMS2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13121-79] to RBPMS2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal liver tissue, PC3 and HepG2 whole cell lysate (ab7900).

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13121-79

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181098の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000. Detects a band of approximately 24, 26 kDa (predicted molecular weight: 22 kDa).
IP
1/50.
特記事項
WB
1/2000. Detects a band of approximately 24, 26 kDa (predicted molecular weight: 22 kDa).
IP
1/50.
追加情報
Is unsuitable for ICC/IF or IHC-P.

ターゲット情報

画像

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    All lanes : Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/2000 dilution (purified)

    Lane 1 : PC-3 lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human fetal liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 24 kDa (possible isoform), 26 kDa (possible isoform)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (purified) + Mouse heart tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (purified) + Rat heart tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 24 kDa (possible isoform), 26 kDa (possible isoform)



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)

    ab181098 (purified) at 1/50 immunoprecipitating RBPMS2 in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    All lanes : Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (unpurified)

    Lane 1 : PC3 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution (unpurified) + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 22 kDa

  • Anti-RBPMS2 antibody [EPR13121-79] (ab181098)
    Anti-RBPMS2 antibody [EPR13121-79] (ab181098)

参考文献 (1)

ab181098 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab181098 は 1 報の論文で使用されています。

  • Dvir S  et al. Uncovering the RNA-binding protein landscape in the pluripotency network of human embryonic stem cells. Cell Rep 35:109198 (2021). PubMed: 34077720

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