Anti-RBPMS 抗体 (ab152101)
Key features and details
- Rabbit polyclonal to RBPMS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RBPMS antibody
RBPMS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RBPMS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 28-202 of Human RBPMS (Q93062; NP_001008712).
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ポジティブ･コントロール
- Wild-type HeLa, A549 and Mouse heart whole cell lysates. IHC-P: Human lung adenocarcinoma tissue. ICC: A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152101の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
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| IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA. -
組織特異性
Ubiquitously expressed, at various levels depending on the isoform and the tissue. -
配列類似性
Contains 1 RRM (RNA recognition motif) domain. -
ドメイン
The RRM domain is necessary for interaction with SMAD4. Both the RRM domain and the C-terminus are required for TGFB1/Smad-mediated transactivation activity. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 614417 Cow
- Entrez Gene: 11030 Human
- Entrez Gene: 19663 Mouse
- Entrez Gene: 498642 Rat
- Omim: 601558 Human
- SwissProt: Q93062 Human
- SwissProt: Q9WVB0 Mouse
- Unigene: 334587 Human
see all -
別名
- FLJ32971 antibody
- Heart and RRM expressed sequence antibody
- Hermes antibody
see all
画像
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Western blot - Anti-RBPMS antibody (ab152101)All lanes : Anti-RBPMS antibody (ab152101) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RBPMS Knockout HeLa cell lysate
Lane 3 : RT-4 cell lysate
Lane 4 : U-251 MG cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab152101 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab152101 was shown to react with RBPMS in wild-type HeLa cells in western blot. The bands observed in RBPMS knockout cell line ab264697 (RBPMS knockout cell lysate ab258631) below 30 kDa may represent truncated forms and cleaved fragments. This has not been investigated further and the functional properties of the gene product have not been determined. HeLa wild-type and RBPMS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab152101 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (ab152101)Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RBPMS with ab152101 at 1/250 dilution. -
Western blot - Anti-RBPMS antibody (ab152101)Anti-RBPMS antibody (ab152101) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 24 kDa
12% SDS PAGE -
Western blot - Anti-RBPMS antibody (ab152101)Anti-RBPMS antibody (ab152101) at 1/1000 dilution + Mouse heart whole cell lysate at 50 µg
Predicted band size: 24 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RBPMS antibody (ab152101)Immunofluorescent analysis of methanol-fixed A549 cells labeling RBPMS with ab152101 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab152101 は 21 報の論文で使用されています。
- Zeng Z et al. Pathologically high intraocular pressure induces mitochondrial dysfunction through Drp1 and leads to retinal ganglion cell PANoptosis in glaucoma. Redox Biol 62:102687 (2023). PubMed: 36989574
- Pérez-Acuña D et al. Retina-to-brain spreading of α-synuclein after intravitreal injection of preformed fibrils. Acta Neuropathol Commun 11:83 (2023). PubMed: 37210559
- Linne C et al. Encephalopsin (OPN3) is required for normal refractive development and the GO/GROW response to induced myopia. Mol Vis 29:39-57 (2023). PubMed: 37287644
- Weigel TK et al. Altered circadian behavior and light sensing in mouse models of Alzheimer's disease. Front Aging Neurosci 15:1218193 (2023). PubMed: 37409006
- Yazdankhah M et al. Mitophagy in Astrocytes Is Required for the Health of Optic Nerve. Cells 12:N/A (2023). PubMed: 37887340