Anti-RBPJK 抗体 (ab25949)
Key features and details
- Rabbit polyclonal to RBPJK
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RBPJK antibody
RBPJK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RBPJK -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human RBPJK.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25949の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use a concentration of 5 µg/ml.
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WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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特記事項 |
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IP
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
ターゲット情報
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機能
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. -
配列類似性
Belongs to the Su(H) family.
Contains 1 IPT/TIG domain. -
細胞内局在
Nucleus. Cytoplasm. Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 3516 Human
- Entrez Gene: 19664 Mouse
- Omim: 147183 Human
- SwissProt: Q06330 Human
- SwissProt: P31266 Mouse
- Unigene: 479396 Human
- Unigene: 209292 Mouse
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別名
- AI843960 antibody
- AOS3 antibody
- CBF 1 antibody
see all
画像
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All lanes : ab25949 at 1 µg/ml
Lane 1 : Testis (Human) Tissue Lysate at 20 µg
Lane 2 : Testis (Mouse) Tissue Lysate at 20 µg
Lane 3 : HeLa (Human) Whole cell lysate at 10 µg
Secondary
All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Anti-RBPJK antibody (ab25949) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 20 µg
Secondary
VHH Single Domain Anti-Rabbit IgG Fc (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25949 overnight at 4°C. Antibody binding was detected using VHH Single Domain Anti-Rabbit IgG Fc (HRP) (ab191866), and visualised using ECL development solution ab133406.
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RBPJK was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to RBPJK and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab25949.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 56kDa, non specific bands -50kDa: This extra band is heavy chain contamination; RBPJK
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Western blot - Anti-RBPJK antibody (ab25949)This image is courtesy of an Abreview submitted by JK FarmahaAll lanes : Anti-RBPJK antibody (ab25949) at 1/500 dilution
Lane 1 : Mouse myoblast C2C12 whole cell lysate at 50 µg
Lane 2 : Mouse myoblast C2C12 whole cell lysate at 75 µg
Secondary
All lanes : HRP-conjugated Goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 1 minute
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (38)
ab25949 は 38 報の論文で使用されています。
- Lee C et al. Epigenetic regulation of Neuregulin 1 promotes breast cancer progression associated to hyperglycemia. Nat Commun 14:439 (2023). PubMed: 36707514
- Yang T et al. Macrophage PTEN controls STING-induced inflammation and necroptosis through NICD/NRF2 signaling in APAP-induced liver injury. Cell Commun Signal 21:160 (2023). PubMed: 37370115
- Pang J et al. NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression. Cell Rep 34:108603 (2021). PubMed: 33440163
- Matsuura N et al. NOTCH3 limits the epithelial-mesenchymal transition and predicts a favorable clinical outcome in esophageal cancer. Cancer Med 10:3986-3996 (2021). PubMed: 34042293
- Pan X et al. LINC01123 enhances osteosarcoma cell growth by activating the Hedgehog pathway via the miR-516b-5p/Gli1 axis. Cancer Sci 112:2260-2271 (2021). PubMed: 33837611