Anti-RBP1 抗体 (ab154881)
Key features and details
- Rabbit polyclonal to RBP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RBP1 antibody
RBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to a region within amino acids 10-103 of Human RBP1 (UniProt ID: P09455).
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ポジティブ･コントロール
- NIH-3T3 and C2C12 whole cell lysates; H1229 cells; Human Colon tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154881の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Intracellular transport of retinol. -
組織特異性
Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver. -
配列類似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
ドメイン
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5947 Human
- Entrez Gene: 19659 Mouse
- Omim: 180260 Human
- SwissProt: P09455 Human
- SwissProt: Q00915 Mouse
- Unigene: 529571 Human
- Unigene: 279741 Mouse
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別名
- Cellular retinol binding protein antibody
- Cellular retinol-binding protein antibody
- Cellular retinol-binding protein I antibody
see all
画像
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Western blot - Anti-RBP1 antibody (ab154881)Anti-RBP1 antibody (ab154881) at 1/5000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE -
Western blot - Anti-RBP1 antibody (ab154881)All lanes : Anti-RBP1 antibody (ab154881) at 1/5000 dilution
Lane 1 : NIH3T3 whole cell lysate
Lane 2 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa
12 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RBP1 antibody (ab154881)Immunofluorescence analysis of methanol fixed H1299 cells, labeling RBP1 using ab154881 at 1/500 dilution. Lower image costained with Hoerchst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (ab154881)Immunohistochemical analysis of paraffin embedded Human colon tissue, labeling RBP1 using ab154881 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab154881 は 6 報の論文で使用されています。
- Zhao Y et al. WITHDRAWN: Circ_0000517 contributes to hepatocellular carcinoma progression by upregulating ARID4B via sponging miR-328-3p. Cell Signal N/A:109950 (2021). PubMed: 33582185
- Hoffmann C et al. Hepatic stellate cell hypertrophy is associated with metabolic liver fibrosis. Sci Rep 10:3850 (2020). PubMed: 32123215
- Sadler E et al. Cell-Specific Transcriptional Responses to Heat Shock in the Mouse Utricle Epithelium. Front Cell Neurosci 14:123 (2020). PubMed: 32528249
- Berner D et al. The protective variant rs7173049 at LOXL1 locus impacts on retinoic acid signaling pathway in pseudoexfoliation syndrome. Hum Mol Genet N/A:N/A (2019). PubMed: 30986821
- Gliniak CM et al. The retinol-binding protein receptor STRA6 regulates diurnal insulin responses. J Biol Chem 292:15080-15093 (2017). PubMed: 28733465
- Zheng Q et al. iTRAQ-Based Proteomic Analysis of Visual Cycle-Associated Proteins in RPE of rd12 Mice before and after RPE65 Gene Delivery. J Ophthalmol 2015:918473 (2015). WB . PubMed: 26124962