Anti-RBM5 抗体 (ab69770)
Key features and details
- Rabbit polyclonal to RBM5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RBM5 antibody
RBM5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RBM5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide derived from an internal region of Human RBM5
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ポジティブ･コントロール
- Brain tissue; extracts from HUVEC cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69770の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis. -
組織特異性
Isoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukemic cell lines. -
配列類似性
Belongs to the RBM5/RBM10 family.
Contains 1 C2H2-type zinc finger.
Contains 1 G-patch domain.
Contains 1 RanBP2-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10181 Human
- Entrez Gene: 83486 Mouse
- Omim: 606884 Human
- SwissProt: P52756 Human
- SwissProt: Q91YE7 Mouse
- Unigene: 439480 Human
- Unigene: 259197 Mouse
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別名
- FLJ39876 antibody
- G15 antibody
- H37 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM5 antibody (ab69770)Left: human brain tissue stained with ab69770 at 1/50 dilution; Right: the antibody was preincubated with the immunizing peptide.
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Western blot - Anti-RBM5 antibody (ab69770)All lanes : Anti-RBM5 antibody (ab69770) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with immunizing peptide at 10 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab69770 は 1 報の論文で使用されています。
- Martínez FP & Tang Q Identification of cellular proteins that interact with human cytomegalovirus immediate-early protein 1 by protein array assay. Viruses 6:89-105 (2014). PubMed: 24385082