AB233147

Anti-RBM20 抗体

Name: Anti-RBM20 抗体 (ab233147) | Abcam
Brand: Abcam Limited
SKU: AB233147
Price: 63000 JPY
Availability: InStock

Anti-RBM20 antibody

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(5 Publications)

Anti-RBM20 antibody (ab233147) is a rabbit polyclonal antibody detecting RBM20 in Western Blot, IHC-P. Suitable for Human, Pig.

別名を表示する

RNA-binding protein 20, RNA-binding motif protein 20, RBM20

3 Images

Supplier Data

Western blot - Anti-RBM20 antibody (AB233147)

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Western blot - Anti-RBM20 antibody (ab233147) at 2 µg/mL

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Recombinant human RBM20 protein

Predicted band size: 134 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM20 antibody (AB233147)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM20 antibody (AB233147)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for RBM20 using ab233147 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-RBM20 antibody (AB233147)

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Western blot - Anti-RBM20 antibody (ab233147) at 1.5 µg/mL

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Pig skeletal muscle lysate

Secondary

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HRP-linked Guinea pig anti-Rabbit at 1/1000 dilution

Predicted band size: 134 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Pig, Human

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human RBM20 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5T481

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-RBM20 antibody (ab233147) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Pig samples.

What is the molecular weight of RBM20?
Anti-RBM20 (ab233147) specifically detects a band for RBM20 (UniProt: Q5T481) at a molecular weight of 134kDa.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

ab233147 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

バッファー組成

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes encoding key structural proteins involved in cardiac development, such as TTN (Titin), CACNA1C, CAMK2D or PDLIM5/ENH (PubMed : 22466703, PubMed : 24960161, PubMed : 26604136, PubMed : 27496873, PubMed : 27531932, PubMed : 29895960, PubMed : 30948719, PubMed : 32840935, PubMed : 34732726, PubMed : 35427468). Acts as a repressor of mRNA splicing : specifically binds the 5'UCUU-3' motif that is predominantly found within intronic sequences of pre-mRNAs, leading to the exclusion of specific exons in target transcripts (PubMed : 24960161, PubMed : 30948719, PubMed : 34732726). RBM20-mediated exon skipping is hormone-dependent and is essential for TTN isoform transition in both cardiac and skeletal muscles (PubMed : 27531932, PubMed : 30948719). RBM20-mediated exon skipping of TTN provides substrates for the formation of circular RNA (circRNAs) from the TTN transcripts (PubMed : 27531932, PubMed : 34732726). Together with RBM24, promotes the expression of short isoforms of PDLIM5/ENH in cardiomyocytes (By similarity).

See full target information RBM20

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular research 121:629-642 PubMed40042822

2025

Antisense-mediated regulation of exon usage in the elastic spring region of Titin modulates sarcomere function.

Applications

Unspecified application

Species

Unspecified reactive species

Selvi Celik,Ludvig Hyrefelt,Tomasz Czuba,Yuan Li,Juliana Assis,Julia Martinez,Markus Johansson,Oscar André,Jane Synnergren,Joakim Sandstedt,Pontus Nordenfelt,Kristina Vukusic,J Gustav Smith,Olof Gidlöf

Asia Pacific allergy 14:97-102 PubMed39220569

2024

Enhanced IL-37-IL-1R8 axis is negatively associated with inflammatory and clinical severity of chronic rhinosinusitis with nasal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhang,Yujie Cao,Kun Chen,Xianting Hu,Chun Zhou,Lei Li,Miaomiao Han,Dehui Wang,Huabin Li

Nature communications 14:4312 PubMed37463913

2023

Mislocalization of pathogenic RBM20 variants in dilated cardiomyopathy is caused by loss-of-interaction with Transportin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Kornienko,Marta Rodríguez-Martínez,Kai Fenzl,Florian Hinze,Daniel Schraivogel,Markus Grosch,Brigit Tunaj,Dominik Lindenhofer,Laura Schraft,Moritz Kueblbeck,Eric Smith,Chad Mao,Emily Brown,Anjali Owens,Ardan M Saguner,Benjamin Meder,Victoria Parikh,Michael Gotthardt,Lars M Steinmetz

Molecular cell 82:2069-2083.e8 PubMed35427468

2022

Proteome-wide quantitative RNA-interactome capture identifies phosphorylation sites with regulatory potential in RBM20.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Henrique Vieira-Vieira,Vita Dauksaite,Anje Sporbert,Michael Gotthardt,Matthias Selbach

Nature communications 12:6324 PubMed34732726

2021

Gain-of-function cardiomyopathic mutations in RBM20 rewire splicing regulation and re-distribute ribonucleoprotein granules within processing bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Aidan M Fenix,Yuichiro Miyaoka,Alessandro Bertero,Steven M Blue,Matthew J Spindler,Kenneth K B Tan,Juan A Perez-Bermejo,Amanda H Chan,Steven J Mayerl,Trieu D Nguyen,Caitlin R Russell,Paweena P Lizarraga,Annie Truong,Po-Lin So,Aishwarya Kulkarni,Kashish Chetal,Shashank Sathe,Nathan J Sniadecki,Gene W Yeo,Charles E Murry,Bruce R Conklin,Nathan Salomonis

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBM20 抗体

Anti-RBM20 antibody

Western blot - Anti-RBM20 antibody (AB233147)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM20 antibody (AB233147)

Western blot - Anti-RBM20 antibody (AB233147)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (5)

Abcam product promise