Anti-Rbm15/OTT 抗体 (ab96544)
Key features and details
- Rabbit polyclonal to Rbm15/OTT
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Rbm15/OTT antibody
Rbm15/OTT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rbm15/OTT -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant fragment corresponding to Human Rbm15/OTT aa 146-395.
Database link: NP_073605 -
ポジティブ･コントロール
- WB: A431, H1299 and Hela cell lystes IHC-P: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96544の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
|
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| ICC/IF |
1/100 - 1/200.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 107 kDa. |
|
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
May be implicated in HOX gene regulation. -
関連疾患
A chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene. -
配列類似性
Belongs to the RRM Spen family.
Contains 3 RRM (RNA recognition motif) domains.
Contains 1 SPOC domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 64783 Human
- Entrez Gene: 229700 Mouse
- Omim: 606077 Human
- SwissProt: Q96T37 Human
- SwissProt: Q6PHZ5 Mouse
- Unigene: 435947 Human
- Unigene: 708172 Human
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別名
- One twenty two protein antibody
- One-twenty two protein 1 antibody
- OTT antibody
see all
画像
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Western blot - Anti-Rbm15/OTT antibody (ab96544)Anti-Rbm15/OTT antibody (ab96544) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 107 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Rbm15/OTT antibody (ab96544)ab96544, at a 1/200 dilution, staining Rbm15/OTT in paraformaldehyde fixed HeLa cells by Immunofluorescence.
Lower image is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab96544 は 2 報の論文で使用されています。
- Ma M et al. The prognostic value of N6-methyladenosine RBM15 regulators in lung adenocarcinoma. Cell Mol Biol (Noisy-le-grand) 68:130-139 (2022). PubMed: 35809319
- Elman JS et al. Identification of FUBP1 as a Long Tail Cancer Driver and Widespread Regulator of Tumor Suppressor and Oncogene Alternative Splicing. Cell Rep 28:3435-3449.e5 (2019). PubMed: 31553912