Anti-Rbm15/OTT 抗体 (ab70549)
Key features and details
- Rabbit polyclonal to Rbm15/OTT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Rbm15/OTT antibody
Rbm15/OTT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rbm15/OTT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70549の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
機能
May be implicated in HOX gene regulation. -
関連疾患
A chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene. -
配列類似性
Belongs to the RRM Spen family.
Contains 3 RRM (RNA recognition motif) domains.
Contains 1 SPOC domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 64783 Human
- Omim: 606077 Human
- SwissProt: Q96T37 Human
- Unigene: 435947 Human
- Unigene: 708172 Human
-
別名
- One twenty two protein antibody
- One-twenty two protein 1 antibody
- OTT antibody
see all
画像
-
Western blot - Anti-Rbm15/OTT antibody (ab70549)All lanes : Anti-Rbm15/OTT antibody (ab70549) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Rbm15/OTT antibody (ab70549)Detection of Human Rbm15/OTT by Western Blot of Immunprecipitate. ab70549 at 1µg/ml staining Rbm15/OTT in HeLa whole cell lysate immunoprecipitated by ab70549 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab70549 は 7 報の論文で使用されています。
- Lim YH et al. Human lncRNA SUGCT-AS1 Regulates the Proinflammatory Response of Macrophage. Int J Mol Sci 24:N/A (2023). PubMed: 37686120
- Lv D et al. PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine. Dev Cell 57:1466-1481.e6 (2022). PubMed: 35659339
- Horiuchi K et al. Wilms' tumor 1-associating protein complex regulates alternative splicing and polyadenylation at potential G-quadruplex-forming splice site sequences. J Biol Chem 297:101248 (2021). PubMed: 34582888
- Wang CY et al. PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments. Nat Commun 10:2950 (2019). PubMed: 31270318
- Jin S et al. PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+cells. Exp Ther Med 15:2563-2568 (2018). PubMed: 29456659
- Knuckles P et al. Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d. Genes Dev 32:415-429 (2018). PubMed: 29535189
- Horiuchi K et al. Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J Biol Chem 288:33292-302 (2013). WB, ICC/IF ; Human . PubMed: 24100041