Anti-Rbm15/OTT + RBM15B 抗体 [EPR25177-138] (BSA and Azide free) (ab300409)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25177-138] to Rbm15/OTT + RBM15B - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (BSA and Azide free) -
製品の詳細
Rabbit monoclonal [EPR25177-138] to Rbm15/OTT + RBM15B - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa transfected with scrambled siRNA control, whole cell lysate; His-tagged human RBM15B and RBM15 recombinant protein; HeLa. 293T, NIH/3T3, PC-12, Raw264.7, Jurkat and 2.4G2 whole cell lysates.
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特記事項
ab300522 is a carrier free version of ab300347.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25177-138 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300409の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa. |
ターゲット情報
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細胞内局在
Rbm15/OTT: Nucleus. RBM15B: Nucleus > nucleoplasm. Colocalizes with BMLF1 in the nucleus. Localized in the nucleoplasm with a granular staining pattern and excluded from the nucleoli. -
参照データベース
- Entrez Gene: 29890 Human
- Entrez Gene: 64783 Human
- Entrez Gene: 109095 Mouse
- Entrez Gene: 229700 Mouse
- Entrez Gene: 315988 Rat
- Entrez Gene: 684233 Rat
- Omim: 606077 Human
- Omim: 612602 Human
see all
画像
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All lanes : Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (ab300347) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lanes 2-3 : HeLa transfected with siRNA specifically targeting RBM15B, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 114 secondsThis data was developed using ab300347, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27602518).
The bands below 100 kDa may be caused by degradation.
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All lanes : Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (ab300347) at 1/1000 dilution
Lane 1 : His-tagged human RBM15B recombinant protein, 10 ng
Lane 2 : His-tagged human RBM15 recombinant protein, 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 97 kDa
Observed band size: 20,25 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsThis data was developed using ab300347, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer: 5% NFDM/TBST.
This antibody reacts with human RBM15 and RBM15B.
Both recombinant proteins were expressed from E.coli expression systems.
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All lanes : Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (ab300347) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThis data was developed using ab300347, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The bands below 100 kDa may be caused by degradation.
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All lanes : Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (ab300347) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : 2.4G2 (Rat B cell lymphoma B lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 92 secondsThis data was developed using ab300347, the same antibody clone in a different buffer formulation.
Blocking / Diluting buffer: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The bands below 100 kDa may be caused by degradation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300409 は論文での使用が確認できていません。