Anti-RBM15B 抗体 [EPR25177-158] (ab300465)

Blocking / Diluting buffer: 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27602518).

The bands below 100 kDa may be caused by degradation.

Blocking / Diluting buffer: 5% NFDM/TBST.

This antibody does not cross-react with human RBM15.

Both recombinant proteins were expressed from E.coli expression systems.

Exposure time 7.75 seconds.

Blocking / Diluting buffer: 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

The bands below 100 kDa may be caused by degradation.

Blocking / Diluting buffer: 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

The bands below 100 kDa may be caused by degradation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cells) labelling RBM15B with ab300465 at 1/500 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line. ab195889. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used as a counterstain at 1/200 dilution (red). The nuclear counterstain is DAPI (blue).

Flow Cytometry (Intracellular) analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling RBM15B (red) with ab300465 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730) Isotype control. Blue - (unlabeled control) - Cells without incubation with primary and secondary antibodies.