Anti-RBM14 抗体 (ab12325)
Key features and details
- Rabbit polyclonal to RBM14
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RBM14 antibody
RBM14 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RBM14 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide within Human RBM14 aa 150-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q96PK6 -
ポジティブ・コントロール
- Nuclear extract from HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12325の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 80 kDa. Prepare working dilutions immediately before use.
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特記事項 |
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IHC-P
1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 80 kDa. Prepare working dilutions immediately before use. |
ターゲット情報
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機能
Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 (PubMed:11443112). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (PubMed:25385835). -
組織特異性
Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. -
配列類似性
Contains 2 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus. Nucleus, nucleolus. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles (PubMed:11790299). Cytoplasmic localization is crucial for its function in suppressing the formation of aberrant centriolar protein complexes (PubMed:25385835). - Information by UniProt
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参照データベース
- Entrez Gene: 100526737 Human
- Entrez Gene: 10432 Human
- Omim: 612409 Human
- SwissProt: Q96PK6 Human
- Unigene: 523822 Human
- Unigene: 533712 Human
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別名
- CoAA antibody
- Coactivator Activator antibody
- Paraspeckle protein 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM14 antibody (ab12325)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling RBM14 with ab12325 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of Human CoAA by Western Blot.
Samples: Nuclear extract (NE) from HeLa cells.
Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human CoAA by Western Blot. Samples: Nuclear extract (NE) from HeLa cells. Antibody: ab12325 used at 0.3 mcg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab12325 は 2 報の論文で使用されています。
- Shiratsuchi G et al. RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34:97-114 (2015). Human . PubMed: 25385835
- Li X et al. Co-activator activator (CoAA) prevents the transcriptional activity of Runt domain transcription factors. J Cell Biochem 108:378-87 (2009). PubMed: 19585539