Anti-Rb (phospho T252) 抗体 [EPR17733] (ab184797)
![Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.co.jp/ps/products/184/ab184797/Images/ab184797-251499-anti-rb-phospho-t252-antibody-epr17733-western-blot.jpg "Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17733] to Rb (phospho T252)
- Suitable for: Dot blot, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Rb (phospho T252) antibody [EPR17733]
Rb 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17733] to Rb (phospho T252) -
由来種
Rabbit -
アプリケーション
適用あり: Dot blot, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: K562 and MCF7 whole cell lysates. IHC-P: Human colon and squamous cell carcinoma of cervix tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17733 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184797の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Dot blot |
1/1000.
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| WB |
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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Dot blot
1/1000. |
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WB
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. -
組織特異性
Expressed in the retina. -
関連疾患
Childhood cancer retinoblastoma
Bladder cancer
Osteogenic sarcoma -
配列類似性
Belongs to the retinoblastoma protein (RB) family. -
ドメイン
The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes. -
翻訳後修飾
Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5925 Human
- Omim: 614041 Human
- SwissProt: P06400 Human
- Unigene: 408528 Human
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別名
- Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
- GOS563 exon 17 substitution mutation causes premature stop antibody
- OSRC antibody
see all
画像
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Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)All lanes : Anti-Rb (phospho T252) antibody [EPR17733] (ab184797) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : K562 whole cell lysate serum starved for 3 days
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 2% BSA /TBST.
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![Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.co.jp/ps/products/184/ab184797/Images/ab184797-251498-anti-rb-phospho-t252-antibody-epr17733-western-blot.jpg)
Western blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)All lanes : Anti-Rb (phospho T252) antibody [EPR17733] (ab184797) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : MCF7 whole cell lysate serum starved for 3 days
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 2% BSA /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.co.jp/ps/products/184/ab184797/Images/ab184797-251497-anti-rb-phospho-t252-antibody-epr17733-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rb (phospho T252) with ab184797 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nuclear staining on sporadic epithelium cells of Human colon is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.co.jp/ps/products/184/ab184797/Images/ab184797-251496-anti-rb-phospho-t252-antibody-epr17733-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Rb (phospho T252) with ab184797 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nuclear staining on tumor cells of Human squamous cell carcinoma of cervix.is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Dot Blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.co.jp/ps/products/184/ab184797/Images/ab184797-251509-anti-rb-phospho-t252-antibody-epr17733-dot-blot.jpg)
Dot Blot - Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)Dot blot analysis of Rb (phospho T252) phospho peptide (Lane 1) and Rb Non-phospho peptide (Lane 2) labeled using ab184797 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody (ab97051) at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)](https://www.abcam.co.jp/ps/products/184/ab184797/Images/ab184797-7-benefits-of-recombinant-antibodies.png)
Anti-Rb (phospho T252) antibody [EPR17733] (ab184797)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab184797 は論文での使用が確認できていません。