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リコンビナントRabMAb

Anti-Rb (phospho S807) 抗体 [EPR17732] (ab184796)

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Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Dot Blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
  • Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17732] to Rb (phospho S807)
  • Suitable for: Dot blot, WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-Rb (phospho S807) antibody [EPR17732]
    Rb 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17732] to Rb (phospho S807)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, WB, ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: K562, MCF7, L-929 and C6 whole cell lysates; Mouse embryo lysate. IHC-P: Human colon, Human squamous cells carcinoma of lung, mouse spleen and rat spleen tissues. ICC/IF: MCF7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184796の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
1/1000.
WB (1)
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
ICC/IF (1)
1/500.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Dot blot
1/1000.
WB
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
ICC/IF
1/500.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

  • 組織特異性

    Expressed in the retina.

  • 関連疾患

    Childhood cancer retinoblastoma
    Bladder cancer
    Osteogenic sarcoma

  • 配列類似性

    Belongs to the retinoblastoma protein (RB) family.

  • ドメイン

    The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.

  • 翻訳後修飾

    Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
    Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
    • GOS563 exon 17 substitution mutation causes premature stop antibody
    • OSRC antibody
    • Osteosarcoma antibody
    • p105-Rb antibody
    • P105RB antibody
    • PP105 antibody
    • pp110 antibody
    • PPP1R130 antibody
    • pRb antibody
    • Prepro retinoblastoma associated protein antibody
    • Protein phosphatase 1 regulatory subunit 130 antibody
    • Rb antibody
    • RB transcriptional corepressor 1 antibody
    • RB_HUMAN antibody
    • RB1 antibody
    • RB1 gene antibody
    • Retinoblastoma 1 antibody
    • Retinoblastoma suspectibility protein antibody
    • Retinoblastoma-associated protein antibody
    see all

画像

  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    All lanes : Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/20000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) serum starved for 44 h. Whole cell lysates
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) serum starved for 24 h. Then 10% FBS incubated for 20 h. Whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 10 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells, Serum starved and non-starved, labeling Rb (phospho S807) with Ab184796 at 1/200 dilution followed by Goat anti-Rabbit secondary IgG AlexaFluor®488 (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing positive staining on MCF7 cells. The number of positive cells increased after treatment with FBS (fetal bovine serum) for 48 hours, then decreased after Lambda Protein Phosphatase treatment (31℃ for 2 hours ).

  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    All lanes : Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/20000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
    Lane 2 : K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates serum starved for 2 days

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    All lanes : Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/5000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
    Lane 2 : K562 (human chronic myelogenous leukemia cells from bone marrow) whole cell lysates treated with Lambda Phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution
    Lane 2 : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/1000 dilution + L-929 (mouse connective tissue fibroblast cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/2000 dilution + Mouse embryo lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/2000 dilution + C6 (rat glial tumor cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Western blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Anti-Rb (phospho S807) antibody [EPR17732] (ab184796) at 1/1000 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on epithelial cells of Human colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

    Immunohistochemical analysis of paraffin-embedded human Squamous cells carcinoma of lung tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on squamous cells carcinoma of lung is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on lymphocytes of mouse spleen is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Rb (phospho S807) with ab184796 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on lymphocytes of rat spleen is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Dot Blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Dot Blot - Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

    Dot blot analysis of Rb (phospho S807) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab184796 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)
    Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

参考文献 (28)

ab184796 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184796 は 28 報の論文で使用されています。

  • Zhu Q  et al. 7-Ketocholesterol accelerates pancreatic β-cell senescence by inhibiting the SIRT1/CDK4-Rb-E2F1 signaling pathway. Islets 15:2219105 (2023). PubMed: 37265106
  • Wang J  et al. Influence of cyclin D1 splicing variants expression on breast cancer chemoresistance via CDK4/CyclinD1-pRB-E2F1 pathway. J Cell Mol Med 27:991-1005 (2023). PubMed: 36915230
  • Zhu X  et al. Carboxypeptidase vitellogenic like facilitates resistance to CDK4/6 inhibitors in breast cancer. Thorac Cancer 14:983-991 (2023). PubMed: 36825764
  • Xing J  et al. Expression of p53 and Rb reveal subtypes of gastric neuroendocrine carcinoma with distinct prognosis. J Neuroendocrinol 35:e13257 (2023). PubMed: 36964649
  • Huang ML & Luo WL Engrailed homeobox 1 transcriptional regulation of COL22A1 inhibits nasopharyngeal carcinoma cell senescence through the G1/S phase arrest. J Cell Mol Med 26:5473-5485 (2022). PubMed: 36196630
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Rb (phospho S807) antibody [EPR17732]

 Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Human Cell (RPE))
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
10 µg
Specification
Human Cell (RPE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Oliva Palander

Verified customer

投稿 Jun 17 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rb (phospho S807) antibody [EPR17732]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

投稿 Jul 03 2018

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