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リコンビナントRabMAb

Anti-Rb (phospho S780) 抗体 [EPR17624] (ab184702)

Submit a review Submit a question References (2)
Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
  • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
  • Dot Blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
  • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
  • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
  • Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
  • Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17624] to Rb (phospho S780)
  • Suitable for: WB, IP, Dot blot
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Rb (phospho S780) antibody [EPR17624]
    Rb 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17624] to Rb (phospho S780)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, Dot blotmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: K562 whole cell lysates untreated and serum starved for 2 days; L-929 whole cell lysate. IP: K562 whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184702の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
IP
1/30.
Dot blot
Use at an assay dependent concentration.
特記事項
WB
1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
IP
1/30.
Dot blot
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

  • 組織特異性

    Expressed in the retina.

  • 関連疾患

    Childhood cancer retinoblastoma
    Bladder cancer
    Osteogenic sarcoma

  • 配列類似性

    Belongs to the retinoblastoma protein (RB) family.

  • ドメイン

    The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes.

  • 翻訳後修飾

    Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
    N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
    Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
      • GOS563 exon 17 substitution mutation causes premature stop antibody
      • OSRC antibody
      • Osteosarcoma antibody
      • p105-Rb antibody
      • P105RB antibody
      • PP105 antibody
      • pp110 antibody
      • PPP1R130 antibody
      • pRb antibody
      • Prepro retinoblastoma associated protein antibody
      • Protein phosphatase 1 regulatory subunit 130 antibody
      • Rb antibody
      • RB transcriptional corepressor 1 antibody
      • RB_HUMAN antibody
      • RB1 antibody
      • RB1 gene antibody
      • Retinoblastoma 1 antibody
      • Retinoblastoma suspectibility protein antibody
      • Retinoblastoma-associated protein antibody
      see all

    画像

    • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/5000 dilution

      Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates prepared in RIPA lysis method
      Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

      Predicted band size: 106 kDa



      The lysates were prepared in 1%SDS Hot lysis method

      Observed MW: 106 kDa

      blocking/diluting buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/1000 dilution

      Lane 1 : Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate serum starved for 2 days

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 106 kDa
      Observed band size: 106 kDa


      Exposure time: 10 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Rb phosphorylation at S780 is regulated by cell cycle progression, which is consistent with what has been described in the literature (PMID: 11134518 and 24765199).

      The lysates were prepared in 1%SDS Hot lysis method

    • Dot Blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      Dot Blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

      Dot blot analysis of Rb (phospho S780) labeled with ab184702 at 1/1000 dilution.

      Lane 1: Rb (phospho S780) phospho peptide;

      Lane 2: Rb non-phospho peptide.

      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 was used as secondary antibody.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/1000 dilution

      Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate treated with Alkaline Phosphatase for 1 hour

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 106 kDa
      Observed band size: 106 kDa


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The lysates were prepared in 1%SDS Hot lysis method

    • Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      Western blot - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      All lanes : Anti-Rb (phospho S780) antibody [EPR17624] (ab184702) at 1/5000 dilution

      Lane 1 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
      Lane 2 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate treated with Alkaline Phosphatase for 1 hour

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 106 kDa
      Observed band size: 106 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The lysates were prepared in 1%SDS Hot lysis method

    • Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

      Rb (phospho S780) was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab184702 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: K562 whole cell lysate,10µg (Input).

      Lane 2: ab184702 IP in K562 whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184702 in K562 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)
      Anti-Rb (phospho S780) antibody [EPR17624] (ab184702)

    参考文献 (2)

    ab184702 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184702 は 2 報の論文で使用されています。

    • Xie Z  et al. C2orf40 inhibits metastasis and regulates chemo-resistance and radio-resistance of nasopharyngeal carcinoma cells by influencing cell cycle and activating the PI3K/AKT/mTOR signaling pathway. J Transl Med 20:264 (2022). PubMed: 35676661
    • Buitrago-Molina LE  et al. p53-Independent Induction of p21 Fails to Control Regeneration and Hepatocarcinogenesis in a Murine Liver Injury Model. Cell Mol Gastroenterol Hepatol 11:1387-1404 (2021). PubMed: 33484913

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