Anti-Rb (phospho S780) 抗体 (ab47763)
Key features and details
- Rabbit polyclonal to Rb (phospho S780)
- Suitable for: ELISA, WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Rb (phospho S780) antibody
Rb 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Rb (phospho S780) -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Rb (phospho S780).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47763の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 106 kDa).
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| IHC-P |
1/50 - 1/100.
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| IHC-Fr |
Use at an assay dependent concentration. PubMed: 17690131
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| 特記事項 |
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ELISA
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 106 kDa). |
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IHC-P
1/50 - 1/100. |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 17690131 |
ターゲット情報
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機能
Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. -
組織特異性
Expressed in the retina. -
関連疾患
Childhood cancer retinoblastoma
Bladder cancer
Osteogenic sarcoma -
配列類似性
Belongs to the retinoblastoma protein (RB) family. -
ドメイン
The Pocket domain binds to the threonine-phosphorylated domain C, thereby preventing interaction with heterodimeric E2F/DP transcription factor complexes. -
翻訳後修飾
Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.
N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1.
Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5925 Human
- Entrez Gene: 19645 Mouse
- Entrez Gene: 24708 Rat
- Omim: 614041 Human
- SwissProt: P06400 Human
- SwissProt: P13405 Mouse
- SwissProt: P33568 Rat
- Unigene: 408528 Human
see all -
別名
- Exon 17 tumor GOS561 substitution mutation causes premature stop antibody
- GOS563 exon 17 substitution mutation causes premature stop antibody
- OSRC antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody (ab47763)
ab47763 at 1/50 dilution staining paraffin-embedded human breast carcinoma; left without and right with immunising peptide.
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Western blot - Anti-Rb (phospho S780) antibody (ab47763)All lanes : Anti-Rb (phospho S780) antibody (ab47763) at 1/500 dilution
Lane 1 : K562 cell extract treated with serum for 20 hours
Lane 2 : K562 cell extract
Predicted band size: 106 kDa
Typically 5-30ug of total protein was loaded per lane of the gel. -
Western blot - Anti-Rb (phospho S780) antibody (ab47763)Western blot analysis of extracts from K562 cells treated or untreated with serum using Rb antibody and ab47763.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (42)
ab47763 は 42 報の論文で使用されています。
- Constantin TA et al. The CDK7 inhibitor CT7001 (Samuraciclib) targets proliferation pathways to inhibit advanced prostate cancer. Br J Cancer 128:2326-2337 (2023). PubMed: 37076563
- Komatsu M et al. Aberrant GIMAP2 expression affects oral squamous cell carcinoma progression by promoting cell cycle and inhibiting apoptosis. Oncol Lett 23:49 (2022). PubMed: 34992682
- Sun Y et al. Context-dependent tumor-suppressive BMP signaling in diffuse intrinsic pontine glioma regulates stemness through epigenetic regulation of CXXC5. Nat Cancer 3:1105-1122 (2022). PubMed: 35915262
- Han Z et al. DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway. Front Oncol 12:1036401 (2022). PubMed: 36408174
- Aschacher T et al. Impacts of Telomeric Length, Chronic Hypoxia, Senescence, and Senescence-Associated Secretory Phenotype on the Development of Thoracic Aortic Aneurysm. Int J Mol Sci 23:N/A (2022). PubMed: 36555139