Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
Related conjugates and formulations
製品の概要
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製品名
Rat IgG1, kappa monoclonal [RTK2071] - Isotype control
Rat アイソタイプ・コントロール 製品一覧 -
アプリケーション
適用あり: Flow Cytmore details -
免疫原
Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.
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特記事項
The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
アイソタイプ・コントロール 備考
The ab18412, RTK2071 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues. -
ポリ/モノ
モノクローナル -
クローン名
RTK2071 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
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別名
- Rat Isotype Control
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18412の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 1µg for 106 cells.
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特記事項 |
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Flow Cyt
Use 1µg for 106 cells. |
画像
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Overlay histogram showing HeLa cells stained with ab16881 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16881, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab18412 は 7 報の論文で使用されています。
- Shirzeyli MH et al. Comparison of the efficacy of bone morphogenetic protein-4 on in vitro differentiation of murine adipose and bone marrow mesenchymal stem cells into primordial germ cells. Res Pharm Sci 17:123-133 (2022). PubMed: 35280837
- Sonzogni O et al. T-SIGn tumor reengineering therapy and CAR T cells synergize in combination therapy to clear human lung tumor xenografts and lung metastases in NSG mice. Oncoimmunology 11:2029070 (2022). PubMed: 35154906
- Ikonomou L et al. The in vivo genetic program of murine primordial lung epithelial progenitors. Nat Commun 11:635 (2020). PubMed: 32005814
- Mohammadian F & Negahdari B Isolation and characterization of mesenchymal stem cells and its antitumor application on ovarian cancer cell line. Artif Cells Nanomed Biotechnol 46:1744-1753 (2018). PubMed: 29199459
- Shirzeyli MH et al. Bones Morphogenic Protein-4 and retinoic acid combined treatment comparative analysis for in vitro differentiation potential of murine mesenchymal stem cells derived from bone marrow and adipose tissue into germ cells. Microsc Res Tech 80:1151-1160 (2017). PubMed: 28921810
- Monfared MH et al. Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells. Iran J Basic Med Sci 19:1186-1192 (2016). PubMed: 27917274
- Raymond AS & Shur BD A novel role for SED1 (MFG-E8) in maintaining the integrity of the epididymal epithelium. J Cell Sci 122:849-58 (2009). PubMed: 19240116