Anti-RASL11A 抗体 (ab180997)
Key features and details
- Rabbit polyclonal to RASL11A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RASL11A antibody -
製品の詳細
Rabbit polyclonal to RASL11A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human RASL11A aa 38-164.
Sequence:VGKSAMIVRFLTKRFIGDYEPNTGKLYSRLVYVEGDQLSLQIQDTPGGVQ IQDSLPQVVDSLSKCVQWAEGFLLVYSITDYDSYLSIRPLYQHIRKVHPD SKAPVIIVGNKGDLLHARQVQTQDGIQ
Database link: Q6T310 -
ポジティブ･コントロール
- Human colon tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS -
Concentration information loading... -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180997の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Regulator of rDNA transcription. Acts in cooperation UBF/UBTF and positively regulates RNA polymerase I transcription. -
組織特異性
Widely expressed. Down-regulated in prostate tumors compared to normal prostate tissue. High levels found in colon tumor and normal colon tissue followed by small intestine, liver, jejunum, ileum, bladder and aorta. Lowest levels observed in endothelial cells. -
配列類似性
Belongs to the small GTPase superfamily. Ras family. -
発生段階
Down-regulated during development of THP-1 monocytes into macrophages. -
細胞内局在
Nucleus > nucleolus. Associates with rDNA transcription unit throughout the cell cycle. - Information by UniProt
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参照データベース
- Entrez Gene: 387496 Human
- Entrez Gene: 68895 Mouse
- Entrez Gene: 304268 Rat
- Omim: 612403 Human
- SwissProt: Q6T310 Human
- SwissProt: Q6IMB1 Mouse
- SwissProt: Q6IMA3 Rat
- Unigene: 192131 Human
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別名
- Ras-like protein family member 11A antibody
- RAS-like, family 11, member A antibody
- Rasl11a antibody
- RSLBA_HUMAN antibody
画像
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Western blot - Anti-RASL11A antibody (ab180997)Lanes 2-3 : Anti-RASL11A antibody (ab180997)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing RASL11A with a C-terminal myc-DDK tag (~3.1kDa) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASL11A antibody (ab180997)Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling RASL11A using ab180997 at a 1/50 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab180997 は論文での使用が確認できていません。