Anti-RASA3 抗体 (ab153846)
Key features and details
- Rabbit polyclonal to RASA3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RASA3 antibody -
製品の詳細
Rabbit polyclonal to RASA3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Xenopus laevis
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免疫原
Recombinant fragment corresponding to Human RASA3 aa 583-834 (C terminal).
Database link: Q14644 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153846の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Detects a band of approximately 96 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Detects a band of approximately 96 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor. -
配列類似性
Contains 1 Btk-type zinc finger.
Contains 2 C2 domains.
Contains 1 PH domain.
Contains 1 Ras-GAP domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 22821 Human
- Entrez Gene: 29372 Rat
- Omim: 605182 Human
- SwissProt: Q14644 Human
- SwissProt: Q9QYJ2 Rat
- Unigene: 593075 Human
- Unigene: 224604 Rat
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別名
- GAP III antibody
- GAP1(IP4BP) antibody
- GAP1IP4BP antibody
see all
画像
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Western blot - Anti-RASA3 antibody (ab153846)This image is courtesy of an anonymous Abreview.All lanes : Anti-RASA3 antibody (ab153846) at 1/400 dilution
Lane 1 : Mouse platelet whole cell lysate
Lane 2 : Human platelet whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 800CW - conjugated Oddyssey goat IgG at 1/15000 dilution
Performed under reducing conditions.
Additional bands at: 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hourBlocking agent: 3% BSA for 60 minutes at 20oC.
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Western blot - Anti-RASA3 antibody (ab153846)Anti-RASA3 antibody (ab153846) at 1/2000 dilution + IMR32 whole cell lysate at 30 µg
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA3 antibody (ab153846)Immunohistochemical analysis of paraffin-embedded Human MCF7 xenograft tissue labeling RASA3 with ab153846 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab153846 は 1 報の論文で使用されています。
- Johansen KH et al. A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1-mediated adhesion in T cells. Sci Signal 15:eabl9169 (2022). PubMed: 35857633