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AB323554

Anti-RASA2 抗体 [EPR29503-39]

Anti-RASA2 antibody [EPR29503-39]

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Rabbit Recombinant Monoclonal RASA2 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples.

別名を表示する

GAP1M, RASGAP, RASA2, Ras GTPase-activating protein 2, GTPase-activating protein 1m, GAP1m

4 Images
Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)
  • WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : skeletal muscle

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

Human thyroid tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 96 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)
  • WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : PC-3

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

DLD-1 (human colorectal adenocarcinoma epithelial cell) whole lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 96 kDa,124 kDa

false

Exposure time: 180s

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)
  • WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1-4 : 92 seconds, lane 5 : 114 seconds

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole lysate at 20 µg

Lane 2:

HFF-1 (human skin fibroblast) whole lysate at 20 µg

Lane 3:

A431 (human epidermoid carcinoma epithelial cell) whole lysate at 20 µg

Lane 4:

NCI-H1299 (human lung carcinoma epithelial cell) whole lysate at 20 µg

Lane 5:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Observed band size: 96 kDa,36 kDa

false

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)
  • WB

Supplier Data

Western blot - Anti-RASA2 antibody [EPR29503-39] (AB323554)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human RASA3 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-RASA2 antibody [EPR29503-39] (ab323554) at 1/1000 dilution

Lane 1:

His-tagged human RASA2 recombinant protein at 10 ng

Lane 2:

His-tagged human RASA3 recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 96 kDa,33 kDa

false

Exposure time: 15s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR29503-39

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4).
See full target information RASA2
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Abcam product promise

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