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AB247875

Anti-RASA1 (phospho Y460) 抗体 [EPR2620(2)] - BSA and Azide free

Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal RASA1 phospho Y460 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

別名を表示する

GAP, RASA, RASA1, Ras GTPase-activating protein 1, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free (AB247875)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free (AB247875)

This data was developed using ab109465, the same antibody clone in a different buffer formulation.ab109465 at 1/100 dilution staining GAP in 293T cells by Immunofluorescence.

Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free (AB247875)
  • WB

Unknown

Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] - BSA and Azide free (AB247875)

This data was developed using ab109465, the same antibody clone in a different buffer formulation.

Note : The lower panel utilized an alternative anti-GAP antibody which does not require phosphorylation for detection of the antigen.

All lanes:

Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (<a href='/products/primary-antibodies/rasa1-phospho-y460-antibody-epr26202-ab109465'>ab109465</a>) at 1/1000 dilution

Lane 1:

293T cell lysates, untreated at 10 µg

Lane 2:

293T cell lysates treated with Pervanadate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 116 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-RASA1 (phospho Y460) antibody [EPR2620(2)]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2620(2)

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody only detects GAP phosphorylated at Tyrosine 460&#46;

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組合せ製品

Primary Antibodies

AB52939

Anti-Ras antibody [EP1125Y]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EP1125Y] (AB52939)

  • 5
  • 12
Primary Antibodies

AB247442

Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FAK (phospho Y576) antibody [EP1897Y] - BSA and Azide free (AB247442)

  • 0
  • 0
Primary Antibodies

AB302940

Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [10-PLC-gamma]

Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [10-PLC-gamma] (AB302940)

  • 0
  • 0
Primary Antibodies

AB109541

Anti-VASP (phospho S156) antibody [EPR1065(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S156) antibody [EPR1065(2)] (AB109541)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

ab247875 is the carrier-free version of ab109465.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

GTPase-activating protein (GAP) that stimulates the intrinsic GTPase activity of Ras proteins, such as NRAS, facilitating their transition from the active GTP-bound state to the inactive GDP-bound state, thereby terminating Ras signaling.
See full target information RASA1 pY460
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

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