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リコンビナントRabMAb

Anti-RASA1 (phospho Y460) 抗体 [EPR2620(2)] (ab109465)

Submit a review Submit a question References (1)
Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)
  • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)
  • Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2620(2)] to RASA1 (phospho Y460)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RASA1 (phospho Y460) antibody [EPR2620(2)]
    RASA1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2620(2)] to RASA1 (phospho Y460)

  • 由来種

    Rabbit

  • 特異性

    ab109465 only detects GAP phosphorylated at Tyrosine 460.

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293T cell lysates; 293T cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR2620(2)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109465の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 116 kDa.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.

  • 組織特異性

    In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).

  • 関連疾患

    Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
    Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
    Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.

  • 配列類似性

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.

  • 翻訳後修飾

    The N-terminus is blocked.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CM AVM antibody
      • CMAVM antibody
      • DKFZp434N071 antibody
      • GAP antibody
      • GTPase activating protein antibody
      • GTPase-activating protein antibody
      • OTTHUMP00000222390 antibody
      • OTTHUMP00000222391 antibody
      • OTTHUMP00000222392 antibody
      • OTTHUMP00000222393 antibody
      • p120GAP antibody
      • p120RASGAP antibody
      • PKWS antibody
      • Ras GTPase-activating protein 1 antibody
      • RAS p21 protein activator (GTPase activating protein) 1 antibody
      • Ras p21 protein activator antibody
      • RASA antibody
      • RASA1 antibody
      • RASA1_HUMAN antibody
      • RasGAP antibody
      • Triphosphatase activating protein antibody
      see all

    画像

    • Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)
      Western blot - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)
      All lanes : Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465) at 1/1000 dilution

      Lane 1 : 293T cell lysates, untreated
      Lane 2 : 293T cell lysates treated with Pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 116 kDa



      Note: The lower panel utilized an alternative anti-GAP antibody which does not require phosphorylation for detection of the antigen.
    • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)
      Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)
      ab109465 at 1/100 dilution staining GAP in 293T cells by Immunofluorescence.
    • Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)
      Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (ab109465)

    参考文献 (1)

    ab109465 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109465 は 1 報の論文で使用されています。

    • Norden PR  et al. Cdc42 and k-Ras Control Endothelial Tubulogenesis through Apical Membrane and Cytoskeletal Polarization: Novel Stimulatory Roles for GTPase Effectors, the Small GTPases, Rac2 and Rap1b, and Inhibitory Influence of Arhgap31 and Rasa1. PLoS One 11:e0147758 (2016). PubMed: 26812085

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