AB40677

Anti-RASA1 抗体 [EP536Y]

Anti-RASA1 antibody [EP536Y]

20ul selling size
RabMAb
Recombinant
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(12 Publications)

Rabbit Recombinant Monoclonal RASA1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 12 publications.

別名を表示する

GAP, RASA, RASA1, Ras GTPase-activating protein 1, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] (AB40677)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] (AB40677)

Immunohistochemical analysis of paraffin embedded human ovary carcinoma using ab40677 diluted 1 : 50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-RASA1 antibody [EP536Y] (AB40677)

All lanes:

Western blot - Anti-RASA1 antibody [EP536Y] (ab40677) at 1/2000 dilution

All lanes:

Rat brain tissue lysate at 10 µg

Predicted band size: 116 kDa

Observed band size: 140 kDa,55 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP536Y

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

GTPase-activating protein (GAP) that stimulates the intrinsic GTPase activity of Ras proteins, such as NRAS, facilitating their transition from the active GTP-bound state to the inactive GDP-bound state, thereby terminating Ras signaling.

See full target information RASA1

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e13204 PubMed36747515

2023

MEG3 sponges miRNA-376a and YBX1 to regulate angiogenesis in ovarian cancer endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yize Li,Lingling Zhang,Yongmei Zhao,Hongyan Peng,Nan Zhang,Wendong Bai

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 30:282-291 PubMed34897876

2021

Identification of the circ_PRKDC/miR-20a-3p/RASA1 axis in regulating HaCaT keratinocyte migration.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Na Jiang,Xiaohui Ji,Wei Liu,Chuanchuan Qi,Xiaomei Zhai

Molecular medicine (Cambridge, Mass.) 27:73 PubMed34238206

2021

CircMTO1 inhibits ox-LDL-stimulated vascular smooth muscle cell proliferation and migration via regulating the miR-182-5p/RASA1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ningning Ji,Yu Wang,Xinyan Gong,Shimao Ni,Hui Zhang

Molecular therapy. Nucleic acids 24:164-174 PubMed33767913

2021

Exosome-transmitted miRNA-335-5p promotes colorectal cancer invasion and metastasis by facilitating EMT via targeting RASA1.

Applications

Unspecified application

Species

Unspecified reactive species

Xuecheng Sun,Feiyan Lin,Wenjing Sun,Weijian Zhu,Daoquan Fang,Lifang Luo,Shuhan Li,Wenqi Zhang,Lei Jiang

Annals of translational medicine 9:104 PubMed33569406

2021

RNA-binding protein QKI suppresses breast cancer via RASA1/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Cao,Chengyu Chu,Xiaoyan Li,Siwen Gu,Qiang Zou,Yiting Jin

Oncology letters 18:6361-6370 PubMed31814846

2019

Colorectal cancer cells differentially impact migration and microRNA expression in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Do Yei Kim,Sang-Soo Lee,Young-Kyung Bae

Molecular medicine reports 19:3393-3402 PubMed30864691

2019

MicroRNA‑4530 suppresses cell proliferation and induces apoptosis by targeting RASA1 in human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Jing,Hong Li,Tao Zhang,Jianxin Lu,Lianjin Zhong

Oncology letters 14:5386-5392 PubMed29098030

2017

Co-expression and significance of Dok2 and Ras p21 protein activator 1 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangrong Huang,Xiaochun Peng,Kun Zhang,Chunyan Li,Bo Su,Yanxiang Zhang,Wangui Yu

Anticancer research 37:781-785 PubMed28179330

2017

Down-regulation of RASA1 Is Associated with Poor Prognosis in Human Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Li Chen,Wei-Chieh Huang,Hsin-Lei Yao,Po-Ming Chen,Ping-Yi Lin,Fu-Yu Feng,Pei-Yi Chu

Scientific reports 6:35308 PubMed27752061

2016

Protein tyrosine phosphatase 1B targets PITX1/p120RasGAP thus showing therapeutic potential in colorectal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Wei Teng,Man-Hsin Hung,Li-Ju Chen,Mao-Ju Chang,Feng-Shu Hsieh,Ming-Hsien Tsai,Jui-Wen Huang,Chih-Lung Lin,Hsiang-Wen Tseng,Zong-Keng Kuo,Jeng-Kai Jiang,Shung-Haur Yang,Chung-Wai Shiau,Kuen-Feng Chen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RASA1 抗体 [EP536Y]

Anti-RASA1 antibody [EP536Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA1 antibody [EP536Y] (AB40677)

Western blot - Anti-RASA1 antibody [EP536Y] (AB40677)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (12)

Abcam product promise