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AB2922

Anti-RASA1 抗体 [B4F8]

Anti-RASA1 antibody [B4F8]

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(7 Publications)

Mouse Monoclonal RASA1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RASA1.

別名を表示する

GAP, RASA, RASA1, Ras GTPase-activating protein 1, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (AB2922)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (AB2922)

ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (AB2922)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (AB2922)

ab2922 labelling GAP (green) in the cytoplasm of Hela cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (AB2922)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (AB2922)

ab2922 labelling GAP (green) in the cytoplasm of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4 °C. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

Western blot - Anti-RASA1 antibody [B4F8] (AB2922)
  • WB

Supplier Data

Western blot - Anti-RASA1 antibody [B4F8] (AB2922)

All lanes:

Western blot - Anti-RASA1 antibody [B4F8] (ab2922) at 1/200 dilution

Lane 1:

HeLa cell lysate at 25 µg

Lane 2:

A431 cell lysate at 25 µg

Lane 3:

Mouse brain cell lysate at 25 µg

Predicted band size: 116 kDa

Observed band size: 110 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

B4F8

アイソタイプ

IgG2a

キャリアフリー

No

交差種

Human, Mouse

アプリケーション

WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human RASA1.

P20936

エピトープ

Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 and SH3.

特異性

This antibody is specific for RASA1 (an alternate name of GAP).

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  • 0
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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p>By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p>Immunofluorescence staining of GAP in mouse fibroblast cells results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane.</p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p>Immunofluorescence staining of GAP in mouse fibroblast cells results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane.</p>" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

GTPase-activating protein (GAP) that stimulates the intrinsic GTPase activity of Ras proteins, such as NRAS, facilitating their transition from the active GTP-bound state to the inactive GDP-bound state, thereby terminating Ras signaling.
See full target information RASA1
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 4:1091 PubMed34531530

2021

TC10 regulates breast cancer invasion and metastasis by controlling membrane type-1 matrix metalloproteinase at invadopodia.

Applications

Unspecified application

Species

Unspecified reactive species

Maren Hülsemann,Colline Sanchez,Polina V Verkhusha,Vera Des Marais,Serena P H Mao,Sara K Donnelly,Jeffrey E Segall,Louis Hodgson

Oncology letters 21:481 PubMed33968197

2021

miR-188-5p promotes oxaliplatin resistance by targeting RASA1 in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xijia Zhu,Xishun Luo,Zhike Song,Shiyu Jiang,Xiangkai Long,Xueyuan Gao,Xinyang Xie,Laijian Zheng,Haipeng Wang

Scientific reports 10:17447 PubMed33060649

2020

KRAS K104 modification affects the KRAS-GEF interaction and mediates cell growth and motility.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Chieh Chen,Chia-Yi Hsu,Hsiao-Yun Lin,Hong-Qi Zeng,Kuang-Hung Cheng,Chia-Wei Wu,Eing-Mei Tsai,Tsung-Hua Hsieh

Neuron 102:602-620.e9 PubMed30902550

2019

p190RhoGAP Filters Competing Signals to Resolve Axon Guidance Conflicts.

Applications

Unspecified application

Species

Unspecified reactive species

Dario Bonanomi,Fabiola Valenza,Onanong Chivatakarn,Matthew J Sternfeld,Shawn P Driscoll,Aaron Aslanian,Karen Lettieri,Miriam Gullo,Aurora Badaloni,Joseph W Lewcock,Tony Hunter,Samuel L Pfaff

Journal of cellular and molecular medicine 19:1994-2005 PubMed25945589

2015

MicroRNA-132/212 family enhances arteriogenesis after hindlimb ischaemia through modulation of the Ras-MAPK pathway.

Applications

IF, WB

Species

Unspecified reactive species, Unspecified reactive species

Zhiyong Lei,Alain van Mil,Maarten M Brandt,Sebastian Grundmann,Imo Hoefer,Michiel Smits,Hamid El Azzouzi,Taro Fukao,Caroline Cheng,Pieter A Doevendans,Joost P G Sluijter

Circulation 124:720-30 PubMed21788589

2011

MicroRNA-24 regulates vascularity after myocardial infarction.

Applications

WB, IHC-Fr

Species

Mouse, Mouse

Jan Fiedler,Virginija Jazbutyte,Bettina C Kirchmaier,Shashi K Gupta,Johan Lorenzen,Dorothee Hartmann,Paolo Galuppo,Susanne Kneitz,John T G Pena,Cherin Sohn-Lee,Xavier Loyer,Juergen Soutschek,Thomas Brand,Thomas Tuschl,Joerg Heineke,Ulrich Martin,Stefan Schulte-Merker,Georg Ertl,Stefan Engelhardt,Johann Bauersachs,Thomas Thum

Cell 69:539-49 PubMed1581965

1992

Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

J Settleman,V Narasimhan,L C Foster,R A Weinberg
View all publications
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