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AB308530

Anti-RASA1 抗体 [13/RAS-GAP]

Anti-RASA1 antibody [13/RAS-GAP]

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Mouse Recombinant Monoclonal RASA1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

別名を表示する

GAP, RASA, RASA1, Ras GTPase-activating protein 1, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP

2 Images
Western blot - Anti-RASA1 antibody [13/RAS-GAP] (AB308530)
  • WB

Supplier Data

Western blot - Anti-RASA1 antibody [13/RAS-GAP] (AB308530)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds.

Lanes 1 - 6:

Western blot - Anti-RASA1 antibody [13/RAS-GAP] (ab308530) at 1/1000 dilution

Lane 4:

Human testis tissue lysate at 20 µg

Lane 5:

Mouse testis tissue lysate at 20 µg

Lane 6:

Rat testis tissue lysate at 20 µg

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 120 kDa

false

Exposure time: 180s

Western blot - Anti-RASA1 antibody [13/RAS-GAP] (AB308530)
  • WB

Supplier Data

Western blot - Anti-RASA1 antibody [13/RAS-GAP] (AB308530)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. In Western blot, Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 81 seconds.

All lanes:

Western blot - Anti-RASA1 antibody [13/RAS-GAP] (ab308530) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

293T transfected with siRNA specifically targeting RASA1 whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 120 kDa

false

Exposure time: 81s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-RASA1 antibody [13/RAS-GAP] - BSA and Azide free

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

13/RAS-GAP

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human, Mouse, Rat

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Primary Antibodies

AB108602

Anti-Ras antibody [EPR3255]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] (AB108602)

  • 5
  • 9
Primary Antibodies

AB254590

Anti-RASA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA2 antibody (AB254590)

  • 0
  • 0
Primary Antibodies

AB109465

Anti-RASA1 (phospho Y460) antibody [EPR2620(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RASA1 (phospho Y460) antibody [EPR2620(2)] (AB109465)

  • 0
  • 0
Primary Antibodies

AB27278

Anti-VEGF Receptor 3 antibody

20ul selling size

Western blot - Anti-VEGF Receptor 3 antibody (AB27278)

  • 4
  • 8
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

GTPase-activating protein (GAP) that stimulates the intrinsic GTPase activity of Ras proteins, such as NRAS, facilitating their transition from the active GTP-bound state to the inactive GDP-bound state, thereby terminating Ras signaling.
See full target information RASA1
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com