Anti-Raptor 抗体 [EP539Y] (ab40768)

Thank you very much for taking the time to complete and send the questionnaire.

The details you have kindly provided will provide us with vital information for our monitoring of product quality.

Unfortunately none of these antibodies have been tested in frozen sections, and therefore they are not guaranteed in this application, nor do we have specific protocols tips to provide for it.

However, there are some suggestions that may optimise the antibody performance, if you wish to give them a try:

-I would recommend decreasing the permeabilization conditions. Both proteins are cytoplasmic; there is no need for such an aggressive permeabilization step. I would repeat the staining skipping this step and removing Triton from the blocking and the washing buffer as well as from the antibody diluent.

-Use milder blocking conditions, using 5% of serum if possible from the same species as the secondary was raised in, to avoid cross reactivity.

-Try using more concentrated dilutions, especially for Raptor antibody (1/50 – 1/25).

If possible I would recommend trying both antibodies in paraffin embedded sections, as they are both guaranteed for it. The link to our protocols page is below, where you can find all the information regarding the different applications protocols, and very useful information for performing paraffin embedded immunostaining.

https://www.abcam.com/index.html?pageconfig=popular_protocols

Should the suggestions not improve the results, please do not hesitate to contact me again and I’ll be more than happy to help you further.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to better understand the problem, I would like to send you a questionnaire with some additional questions about the protocol used, as well as the order details. All this information is crucial to help us understand the possible causes of the problem. Any images are highly appreciated.

Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. If no suggestions can be made to the protocol, and the antibody was purchased within the last 6 months, it is covered by our Abpromise guarantee, and therefore I can offer you a free of charge replacement or a credit note for it.

I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam regarding ab40768. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Thank you for contacting us. The secondary antibodies can be used within range from 1/1000 to 1/50,000 dilution. The dilution range depends on the concentration of secondary antibody. We recommend starting with 1/1000, 1/2000, 1/5000 and 1/10,000 for optimization purpose. The dilution factor should also be recommended on vendor's datasheets of secondary antibodies which will give you more idea at what dilution to use. We use BSA in our own lab. You can use milk however if the background will be high then we may recommend switching to BSA. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.