Anti-RAP1B 抗体 (ab154756)
Key features and details
- Rabbit polyclonal to RAP1B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RAP1B antibody
RAP1B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RAP1B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
-
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-156 of Human RAP1B (UniProt: P61224).
-
ポジティブ･コントロール
- HCT116 whole cell lysate; Human lung adenocarcinoma tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154756の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 20 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) .
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 20 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) . |
ターゲット情報
-
機能
GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. -
配列類似性
Belongs to the small GTPase superfamily. Ras family. -
細胞内局在
Cell membrane. Cytoplasm > cytosol. Cell junction. May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction. - Information by UniProt
-
参照データベース
- Entrez Gene: 417840 Chicken
- Entrez Gene: 327708 Cow
- Entrez Gene: 5908 Human
- Entrez Gene: 215449 Mouse
- Entrez Gene: 171337 Rat
- Entrez Gene: 379883 Xenopus laevis
- Entrez Gene: 493557. Xenopus tropicalis
- Entrez Gene: 321107 Zebrafish
see all -
別名
- GTP-binding protein smg p21B antibody
- K-REV antibody
- OK/SW-cl.11 antibody
see all
画像
-
Western blot - Anti-RAP1B antibody (ab154756)Anti-RAP1B antibody (ab154756) at 1/500 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 20 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP1B antibody (ab154756)Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RAP1B with ab154756 at 1/100 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab154756 は 3 報の論文で使用されています。
- Zhang W et al. lncRNA DLEU2 Accelerates Oral Cancer Progression via miR-30a-5p/RAP1B Axis to Regulate p38 MAPK Signaling Pathway. Dis Markers 2022:9310048 (2022). PubMed: 36277988
- Yao J et al. M2 macrophage-derived exosomal microRNAs inhibit cell migration and invasion in gliomas through PI3K/AKT/mTOR signaling pathway. J Transl Med 19:99 (2021). PubMed: 33676540
- Shi Q et al. Circ6401, a novel circular RNA, is implicated in repair of the damaged endometrium by Wharton's jelly-derived mesenchymal stem cells through regulation of the miR-29b-1-5p/RAP1B axis. Stem Cell Res Ther 11:520 (2020). PubMed: 33261656