Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-RAP1A + RAP1B 抗体 [EP879Y] (ab75871)

Reviews (3) Submit a question References (3)
Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)
  • Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)
  • Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP879Y] to RAP1A + RAP1B
  • Suitable for: WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RAP1A + RAP1B antibody [EP879Y]
    RAP1A + RAP1B 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP879Y] to RAP1A + RAP1B

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt or IHC-P

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human brain lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP879Y

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75871の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
特記事項
WB
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
追加情報
Is unsuitable for Flow Cyt or IHC-P.

ターゲット情報

  • 関連性

    RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known.

  • 細胞内局在

    Cell Membrane; Attached to the membrane by a lipid anchor

  • 参照データベース

    see all

  • 別名

    • C21KG antibody
    • G 22K antibody
    • GTP binding protein smg p21A antibody
    • GTP binding protein smg p21B antibody
    • K REV antibody
    • KREV 1 antibody
    • KREV antibody
    • KREV1 antibody
    • RAL 1B antibody
    • RAL1B antibody
    • RAP 1 antibody
    • RAP 1A antibody
    • RAP 1B antibody
    • RAP1 antibody
    • RAP1A member of RAS oncogene family antibody
    • RAP1B antibody
    • RAP1B member of RAS oncogene family antibody
    • Ras family small GTP binding protein RAP1B antibody
    • RAS related protein 1b antibody
    • Ras related protein Krev 1 antibody
    • Ras related protein Krev1 antibody
    • Ras related protein RAP 1A antibody
    • Ras related protein RAP 1B antibody
    • RAS related protein RAP1A antibody
    • Ras related protein RAP1B antibody
    • small GTP binding protein antibody
    • SMGP 21 antibody
    • SMGP21 antibody
    see all

画像

  • Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)
    Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)
    Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/500 dilution + human brain lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Secondary antibody - goat anti-rabbit HRP (ab6721)

  • Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)
    Western blot - Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)
    Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/1000 dilution + Mouse brain

    Predicted band size: 21 kDa



    Secondary antibody - goat anti-rabbit HRP (ab6721)

  • Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)
    Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871)

参考文献 (3)

ab75871 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab75871 は 3 報の論文で使用されています。

  • Cho EA  et al. Phosphorylation of RIAM by src promotes integrin activation by unmasking the PH domain of RIAM. Structure 29:320-329.e4 (2021). PubMed: 33275877
  • Xu B  et al. GABAB receptor mediate hippocampal neuroinflammation in adolescent male and female mice after cold expose. Brain Res Bull 142:163-175 (2018). PubMed: 30031816
  • Todorovic V  et al. Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation. Mol Biol Cell 25:3749-64 (2014). PubMed: 25208567

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-RAP1A + RAP1B antibody [EP879Y]

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEPG2)
Specification
HEPG2
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 18 2015

Western blot abreview for Anti-RAP1A + RAP1B antibody [EP879Y]

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEPG2
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 13 2015

Western blot abreview for Anti-RAP1A + RAP1B antibody [EP879Y]

 Poor
Abreviews
abreview image
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 15 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com