Anti-RANK 抗体 [64C1385] (ab13918)

Thank you for your enquiry.

All tested applications and species covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

Therefore, I can confirm that RANK antibody ab13918 is indeed tested and covered by the guarantee for both IHC-P and flow cytometry.

RUNX2 antibody ab102712
This is tested for IHC-P (paraffin embedded sections) as stated on the datasheet. However, I am sorry to confirm this has not been tested in flow cytometry.

The following RUNX2 antibody is tested in flow cytometry, I hope this may be of interest to your customer. However, please note this has not been tested in IHC and you will also need to check which species the customer is using. This has been tested and covered by the guarantee for human samples only.

ab135674 RUNX2 antibody
Tested in: Flow Cyt, WB
Reacts with: Human
https://www.abcam.com/RUNX2-antibody-ab135674.html (or use the following: https://www.abcam.com/RUNX2-antibody-ab135674.html).


RANKL antibody ab45039
This is tested for IHC-P (paraffin embedded sections) as stated on the datasheet. However, I am sorry to confirm this has not been tested in flow cytometry.

We do have the following RANKL antibodies tested in flow cytometry. Again, please note that these are not tested in IHC and please also check the species the customer is using:

ab12125 RANKL antibody [12A380]
Tested in: Flow Cyt, WB
Reacts with: Human, Mouse
https://www.abcam.com/RANKL-antibody-12A380-ab12125.html (or use the following: https://www.abcam.com/RANKL-antibody-12A380-ab12125.html).

ab95720 RANKL antibody [IK22/5] (Biotin)
Tested in: Flow Cyt
Reacts with: Mouse
https://www.abcam.com/Biotin-RANKL-antibody-IK225-ab95720.html (or use the following: https://www.abcam.com/Biotin-RANKL-antibody-IK225-ab95720.html).

ab93553 RANKL antibody [IK22/5] (Phycoerythrin)
Tested in: Flow Cyt
Reacts with: Mouse
https://www.abcam.com/PE-RANKL-antibody-IK225-ab93553.html (or use the following: https://www.abcam.com/PE-RANKL-antibody-IK225-ab93553.html).

BMP-7 antibody ab56023
This is tested for IHC-P (paraffin embedded sections). However, I am sorry to confirm this has not been tested in flow cytometry.

We do have the following BMP-7 antibody tested in flow cytometry. Again, please note that this is unfortunately not tested in IHC and please also check the species the customer is using:

ab84030 BMP7 antibody (Biotin)
Tested in: ELISA, Flow Cyt, sELISA, WB
Reacts with: Human, Chinese Hamster
https://www.abcam.com/BMP7-antibody-Biotin-ab84030.html (or use the following: https://www.abcam.com/BMP7-antibody-Biotin-ab84030.html).

Osteoprotegerin (OPG) antibody ab73400
This is tested for IHC-P (paraffin embedded sections). However, I am sorry to confirm this has not been tested in flow cytometry.

The following Osteoprotegerin antibody is tested in both IHC-P and flow cytometry. Please also check the species the customer is using to ensure it is suitable:

ab105935 Osteoprotegerin antibody [ 5G2]
Tested in: ELISA, Flow Cyt, ICC, ICC/IF, IHC-P, WB
Reacts with: Human
https://www.abcam.com/Osteoprotegerin-antibody-5G2-ab105935.html (or use the following: https://www.abcam.com/Osteoprotegerin-antibody-5G2-ab105935.html).

If your customer would like to test any of these antibodies in a previously untested species or application, please do not hesitate to contact us again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Thank you for your response.

The identities of ab13918 with canine were 81%, with 89% positives and some gaps. This is generally the absolute limit for what we consider to be effective. I chose to recommend ab22106 because it was created with a shorter immunogen which shared similar identies. However the differences between the canine and immunogen are at the very end residues whereas the the differences with ab13918 are scattered throughout.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for your reply. I am pleased to provide the following information: We have two RANK antibodies tested flow cytometry, human and mouse (not IHC or rat): ab45092 RANK antibody [9A725] (Phycoerythrin) Mouse monoclonal Tested applications: Flow Cyt Reacts with: Human, Mouse  Click here (or use the following: https://www.abcam.com/RANK-antibody-9A725-PE-ab45092.html). ab13918 RANK antibody [64C1385] Mouse monoclonal Applications: Flow Cyt, IP, WB Reacts with: Human, Mouse  Click here (or use the following: https://www.abcam.com/RANK-antibody-64C1385-ab13918.html). One antibody tested in IHC-P, mouse and human (not rat or flow cytometry):   ab45039 Anti-RANKL antibody [12A668] Mouse monoclonal Tested applications: ICC/IF, IHC-P, WB Reacts with: Human, Mouse Click here (or use the following: https://www.abcam.com/RANKL-antibody-12A668-BSA-and-Azide-free-ab45039.html). I am sorry to confirm we have no RANK antibodies tested in rat. Regrettably I am not able to find the correct rat sequence on the protein database sites, and so am not able to check the alignments with rat.  If you have access to the rat sequence, I can suggest to try alignment to the immunogen for these antibodies. We recommend alignment should be over 85% to predict that an antibody will detect in a different species. I am sorry we also do not have an antibody tested and guaranteed in both IHC and flow cytometry. If you would like to test one of the antibodies in one of these untested applications, we would be able to provide a testing discount offer. Please let me know if you are interested, and I will be pleased to forward the details. This also applies to the rat species. If the alignment is over 70% , we would be able to provide a testing discount for you to try in rat. I am sorry we do not have an antibody that is tested to meet all your requirements on this occasion, but I hope these ones will still be of interest to you.  If you have any further questions, or are interested in the testing discount, please do not hesitate to contact me.

Thank you for your email. I have instructed our accounting department to issue a refund for your order for ab13918. If you have any questions regarding the refund, please contact accounts@abcam.com If I can be of additional assistance, just let me know. Have a nice weekend!

Thank you for your email and patience. I enquired with the originator of this antibody and evidently RANK is a tricky protein to detect and depending upon how the RAW cells were cultured, you may or may not see a signal. However, I would expect that with a dilution as high as 1:100 and incubation overnight at 4C (which you said that you tried) that you would see something. There have not been any problems with this lot reported, but I can offer to send a free of charge replacement vial from a newer batch. Alternatively, I can offer a refund or credit note. Please let me know how you would like to proceed.

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab13918 in Western blotting. I do need a few more details in order to trouble-shoot this: How much protein did you load? Did you use reducing conditions? How were your samples prepared? What did you block with? Is the secondary antibody working with other primaries? Did you check that the protein transferred properly to the membrane? Also, what was the purchase order number or Abcam reference number that was used when you ordered this antibody? Thank you, and I look forward to hearing from you.