Anti-RanGAP1 抗体 (ab2081)
Key features and details
- Goat polyclonal to RanGAP1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RanGAP1 antibody
RanGAP1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RanGAP1 -
由来種
Goat -
特異性
There is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide: ASEDIAKLAETLAK, corresponding to N terminal amino acids 2-15 of Human RanGAP1.
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ポジティブ･コントロール
- 3T3 lysate.
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特記事項
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2081の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa.
A 1 hour primary incubation is recommended for this product. Detects a band of approximately 70 kDa in human brain lysates and 3T3 lysates (predicted MW of 68kDa according to NP_002874). |
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| ICC/IF |
1/500.
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| 特記事項 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 70 kDa. A 1 hour primary incubation is recommended for this product. Detects a band of approximately 70 kDa in human brain lysates and 3T3 lysates (predicted MW of 68kDa according to NP_002874). |
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ICC/IF
1/500. |
ターゲット情報
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機能
GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. -
組織特異性
Highly expressed in brain, thymus and testis. -
配列類似性
Belongs to the RNA1 family.
Contains 6 LRR (leucine-rich) repeats. -
翻訳後修飾
Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis.
Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9. -
細胞内局在
Cytoplasm. Nucleus membrane. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle pole. Cytoplasmic during interphase. Targeted to the nuclear rim after sumoylation. During mitosis, associates with mitotic spindles. Association with kinetochores appears soon after nuclear envelope breakdown and persists until late anaphase. Mitotic location also requires sumoylation. - Information by UniProt
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参照データベース
- Entrez Gene: 5905 Human
- Entrez Gene: 19387 Mouse
- Omim: 602362 Human
- SwissProt: P46060 Human
- SwissProt: P46061 Mouse
- Unigene: 183800 Human
- Unigene: 270975 Mouse
- Unigene: 475271 Mouse
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別名
- Fug 1 antibody
- Fug1 antibody
- GTPase-activating protein, RAN, 1 antibody
see all
画像
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Western blot - Anti-RanGAP1 antibody (ab2081)ab2081 at a concentration of 0.2
µ g/ml staining approximately 70kda RanGAP1 in 3T3 whole cell lysate (RIPA buffer, 35µ g total protein per lane) by Western blot (ECL). Primary incubation = 1 hour. ab2081 at a concentration of 0.2µg/ml staining approximately 70kda RanGAP1 in 3T3 whole cell lysate (RIPA buffer, 35µg total protein per lane) by Western blot (ECL). Primary incubation = 1 hour. -
Immunocytochemistry/ Immunofluorescence - Anti-RanGAP1 antibody (ab2081)Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, Italyab2081 (1/500) detecting RanGap1 in mitotic and interphase Hela cells (green). Dapi was used as counterstain in order to highlight the nucleus (blue) and cells were fixed both in Methanol (-20 °C/ 6 mins, followed by 3 washes in PBS) or Paraformaldehyde (3,7% PFA /10 min at room temperature, 0,1% Triton/PBS 5 min, glycine 5 min, followed by 3 washes in PBS); This antibody also gave good results in NIH-3T3 cells under both the above mentioned fixation procedures (data not shown).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab2081 は 3 報の論文で使用されています。
- Zhang S et al. The ataxin-1 interactome reveals direct connection with multiple disrupted nuclear transport pathways. Nat Commun 11:3343 (2020). PubMed: 32620905
- Barbato S et al. Karyopherin enrichment at the nuclear pore complex attenuates Ran permeability. J Cell Sci 133:N/A (2020). PubMed: 31932502
- He X et al. Characterization of the loss of SUMO pathway function on cancer cells and tumor proliferation. PLoS One 10:e0123882 (2015). PubMed: 25860128