AB2081

Anti-RanGAP1 抗体

Name: Anti-RanGAP1 抗体 (ab2081) | Abcam
Brand: Abcam Limited
SKU: AB2081
Price: 75000 JPY
Availability: InStock

Anti-RanGAP1 antibody

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(3 Publications)

Goat Polyclonal RanGAP1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RANGAP1 aa 1-50.

別名を表示する

KIAA1835, SD, RANGAP1, Ran GTPase-activating protein 1, RanGAP1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-RanGAP1 antibody (AB2081)

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-RanGAP1 antibody (AB2081)

ab2081 (1/500) detecting RanGap1 in mitotic and interphase Hela cells (green). Dapi was used as counterstain in order to highlight the nucleus (blue) and cells were fixed both in Methanol (-20 °C/ 6 mins, followed by 3 washes in PBS) or Paraformaldehyde (3,7% PFA /10 min at room temperature, 0,1% Triton/PBS 5 min, glycine 5 min, followed by 3 washes in PBS); This antibody also gave good results in NIH-3T3 cells under both the above mentioned fixation procedures (data not shown).

Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, Italy

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human RANGAP1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P46060

特異性

There is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. Detects a band of approximately 70 kDa in human brain lysates and 3T3 lysates (predicted MW of 68kDa according to NP_002874).</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RanGAP1 also known as Ran GTPase-activating protein 1 plays an important role in regulating nucleocytoplasmic transport. Mechanically it converts RanGTP to RanGDP in the cytoplasm which is essential for the recycling of Ran and maintenance of the Ran gradient across the nuclear envelope. The molecular mass of RanGAP1 is approximately 70 kDa. RanGAP1 is expressed in various cell types with significant presence in the cytoplasm due to its function.

Biological function summary

Many cellular processes rely on the action of RanGAP1 as it forms part of the Ran cycle. This protein is an integral component of the nuclear transport complex which regulates the bidirectional movement of RNA and proteins through the nuclear pores. Through its action RanGAP1 indirectly influences nuclear import and export playing an essential part in cell cycle regulation and mitosis.

Pathways

Several key cellular pathways are dependent on RanGAP1 activity. For instance it is a component of the mitotic spindle assembly pathway and also plays a role in the RNA processing pathway. In these pathways RanGAP1 works closely with other proteins such as RanBP1 and RCC1 facilitating important cellular events that require strict regulation of nuclear-cytoplasmic transport.

Disruptions in RanGAP1 function have been associated with certain types of cancer and neurodegenerative diseases. Aberrant regulation of RanGAP1 activity can lead to improper cell cycle progression contributing to oncogenesis. Furthermore changes in the expression of RanGAP1 and its interacting proteins like RanBP2 may relate to disorders like Huntington's disease where cellular transport mechanisms are compromised.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

GTPase activator for RAN (PubMed : 16428860, PubMed : 8146159, PubMed : 8896452). Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed : 27160050, PubMed : 8896452). Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed : 27160050).

See full target information RANGAP1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:3343 PubMed32620905

2020

The ataxin-1 interactome reveals direct connection with multiple disrupted nuclear transport pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sunyuan Zhang,Nicholas A Williamson,Lisa Duvick,Alexander Lee,Harry T Orr,Austin Korlin-Downs,Praseuth Yang,Yee-Foong Mok,David A Jans,Marie A Bogoyevitch

Journal of cell science 133: PubMed31932502

2020

Karyopherin enrichment at the nuclear pore complex attenuates Ran permeability.

Applications

Unspecified application

Species

Unspecified reactive species

Suncica Barbato,Larisa E Kapinos,Chantal Rencurel,Roderick Y H Lim

PloS one 10:e0123882 PubMed25860128

2015

Characterization of the loss of SUMO pathway function on cancer cells and tumor proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyue He,Jessica Riceberg,Sai M Pulukuri,Steve Grossman,Vaishali Shinde,Pooja Shah,James E Brownell,Larry Dick,John Newcomb,Neil Bence

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RanGAP1 抗体

Anti-RanGAP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RanGAP1 antibody (AB2081)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise